Rabbit Polyclonal Phospho-Cdc25C (T48) antibody (STJ90848)

$99.00$319.00

Reactivity: Human
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Cdc25C (T48) antibody (STJ90848)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cdc25C (phospho Thr48) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CDC25C antibody,|CDC 25 antibody|Cdc 25C antibody|CDC25 antibody|CDC25C antibody|Cell division cycle 25 homolog C antibody|Cell division cycle 25C antibody|Cell division cycle 25C protein antibody|Dual specificity phosphatase Cdc25C antibody|M phase inducer phosphatase 3 antibody|M-phase inducer phosphatase 3 antibody|Mitosis inducer CDC25 antibody|MPIP3 antibody|MPIP3_HUMAN antibody|Phosphotyrosine phosphatase antibody|PPP1R60 antibody|protein phosphatase 1, regulatory subunit 60 antibody|Anti-Cdc25C antibody [E302] (ab32444)
SCBT cat No: sc-327|sc-55513|sc-5620|sc-13138|sc-56269|sc-377338|

 

Name

Cdc25C (phospho Thr48) Polyclonal Antibody

Catalogue No.

STJ90848

Reactivity

Human

Specificity

Phospho-Cdc25C (T48) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at T48.

Immunogen

Synthesized phospho-peptide derived from Cdc25C (phospho Thr48) at AA range 30-110

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

80 kDa

Formulation

Cdc25C (phospho Thr48) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cdc25C (phospho Thr48) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

M-phase inducer phosphatase 3 antibody, Dual specificity phosphatase Cdc25C antibody

Database links

Human UniProt/Swiss-Prot:P30307;Mouse UniPort/Swiss-Prot: P48967;Rat UniProt/Swiss-Port: D4ADT2;Human Entrez Gene: 995;Mouse Entrez Gene: 12532;Rat Entrez Gene: Rn.162298

Protein names

M-phase inducer phosphatase 3 , Dual specificity phosphatase Cdc25C

Protein function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. / Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Protein tissue specificity

Expressed predominantly in G2 phase.

Protein sequence and domain

Belongs to the MPI phosphatase family. / Contains 1 rhodanese domain.

Protein post-translational modifications

Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216 . However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.

Protein cellular localization

Nucleus

Research area

All research areas>Cell Cycle Proteins>Cdc
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize Cdc25C (phospho Thr48) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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