Rabbit Polyclonal Phospho-Cdk7 (T170) antibody (STJ90891)

$99.00$319.00

Reactivity: Human, Mouse
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Cdk7 (T170) antibody (STJ90891)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cdk7 (phospho Thr170) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CDK7 antibody, CAK antibody, CAK1 antibody, CDKN7 antibody, MO15 antibody, STK1 antibody,|39 kDa protein kinase antibody|CAK antibody|CAK1 antibody|Cdk activating kinase antibody|CDK-activating kinase 1 antibody|CDK-activating kinase antibody|cdk7 antibody|CDK7_HUMAN antibody|CDKN7 antibody|Cell division protein kinase 7 antibody|Cyclin dependent kinase 7 antibody|cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase) antibody|Cyclin-dependent kinase 7 antibody|HCAK antibody|Homolog of Xenopus MO15 Cdk activating kinase antibody|Kinase subunit of CAK antibody|MO15 antibody|MO15, Xenopus, homolog of antibody|P39 Mo15 antibody|p39MO15 antibody|Serine threonine kinase Stk1 antibody|Serine/threonine protein kinase 1 antibody|Serine/threonine protein kinase MO15 antibody|STK1 antibody|TFIIH basal transcription factor complex kinase subunit antibody|Phospho anti-Cdk7 (T170) antibody [EPR6650(2)] (ab155976)
SCBT cat No: sc-136386|sc-529|sc-7344|sc-365075|sc-271794|sc-856|sc-393308|sc-56284|sc-723|sc-1521|

 

Name

Cdk7 (phospho Thr170) Polyclonal Antibody

Catalogue No.

STJ90891

Reactivity

Human, Mouse

Specificity

Phospho-Cdk7 (T170) Polyclonal Antibody detects endogenous levels of Cdk7 protein only when phosphorylated at T170.

Immunogen

Synthesized phospho-peptide derived from Cdk7 (phospho Thr170) at AA range 110-190

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

40 kDa

Formulation

Cdk7 (phospho Thr170) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cdk7 (phospho Thr170) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cyclin-dependent kinase 7 antibody, 39 kDa protein kinase antibody, p39 Mo15 antibody, CDK-activating kinase 1 antibody, Cell division protein kinase 7 antibody, Serine/threonine-protein kinase 1 antibody, TFIIH basal transcription factor complex kinase subunit antibody

Database links

Human UniProt/Swiss-Prot:P50613;Mouse UniPort/Swiss-Prot: Q03147;Human Entrez Gene: 1022;Mouse Entrez Gene: 12572;Rat Entrez Gene: Rn.98896

Protein names

Cyclin-dependent kinase 7 , 39 kDa protein kinase , p39 Mo15 , CDK-activating kinase 1 , Cell division protein kinase 7 , Serine/threonine-protein kinase 1 , TFIIH basal transcription factor complex kinase subunit

Protein function

Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition. / ATP + a protein = ADP + a phosphoprotein. / ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. / Inactivated by phosphorylation. Repressed by roscovitine (seliciclib, CYC202), R547 (Ro-4584820) and SNS-032 (BMS-387032). The association of p53/TP53 to the CAK complex in response to DNA damage reduces kinase activity toward CDK2 and RNA polymerase II repetitive C-terminal domain (CTD), thus stopping cell cycle progression. The inactivation by roscovitine promotes caspase-mediated apoptosis in leukemic cells.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation of Ser-164 during mitosis inactivates the enzyme. Phosphorylation of Thr-170 is required for activity. Phosphorylated at Ser-164 and Thr-170 by CDK2.

Protein cellular localization

Nucleus / Cytoplasm / Cytoplasm > perinuclear region

Research area

All research areas>Cell Cycle Proteins>Cdk
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize Cdk7 (phospho Thr170) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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