Rabbit Polyclonal Phospho-Cdk9 (T186) antibody (STJ90525)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-Cdk9 (T186) antibody (STJ90525)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cdk9 (phospho Thr186) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

CDK9 antibody, CDC2L4 antibody, TAK antibody,|C-2K antibody|CDC2L4 antibody|Cdk 9 antibody|Cdk9 antibody|CDK9_HUMAN antibody|Cell division cycle 2-like protein kinase 4 antibody|Cell division protein kinase 9 antibody|CTK1 antibody|Cyclin dependent kinase 9 antibody|Cyclin-dependent kinase 9 antibody|PITALRE antibody|Serine/threonine-protein kinase PITALRE antibody|TAK antibody|Tat associated kinase complex catalytic subunit antibody|Anti-Cdk9 antibody (ab6544)
SCBT cat No: sc-484|sc-13130|sc-376624|sc-376646|sc-393422|sc-8338|sc-7331|sc-135456|

 

Name

Cdk9 (phospho Thr186) Polyclonal Antibody

Catalogue No.

STJ90525

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Cdk9 (T186) Polyclonal Antibody detects endogenous levels of Cdk9 protein only when phosphorylated at T186.

Immunogen

Synthesized phospho-peptide derived from Cdk9 (phospho Thr186) at AA range 130-210

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

42 kDa

Formulation

Cdk9 (phospho Thr186) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cdk9 (phospho Thr186) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cyclin-dependent kinase 9 antibody, C-2K antibody, Cell division cycle 2-like protein kinase 4 antibody, Cell division protein kinase 9 antibody, Serine/threonine-protein kinase PITALRE antibody, Tat-associated kinase complex catalytic subunit antibody

Database links

Human UniProt/Swiss-Prot:P50750;Mouse UniPort/Swiss-Prot: Q99J95;Rat UniProt/Swiss-Port: Q641Z4;Human Entrez Gene: 1025;Mouse Entrez Gene: 107951;Rat Entrez Gene: Rn.98228

Protein names

Cyclin-dependent kinase 9 , C-2K , Cell division cycle 2-like protein kinase 4 , Cell division protein kinase 9 , Serine/threonine-protein kinase PITALRE , Tat-associated kinase complex catalytic subunit

Protein function

Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on ‘Ser-2′ in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation. / ATP + a protein = ADP + a phosphoprotein. / ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. / Inhibited by CDKI-71, CR8, GPC-286199, AG-024322, flavopiridol (alvocidib), RBG-286147, anilinopyrimidine 32, arylazopyrazole 31b, indirubin 3’-monoxime, meriolin 3,P276-00, olomoucine II, pyrazolotriazine, meriolin, variolin, thiazolyl-pyrimidine, thiazolyl-pyrimidine, indirubin-30-monoxime, ZK 304709, AG-012986, AT7519, R547, RGB-286638, imidazole pyrimidine, EXEL-3700, EXEL-8647, 5,6-dichloro-1-b-ribofur-anosyl-benzimidazole (DRB), P276-00, roscovitine (seliciclib, CYC202) and SNS-032 (BMS-387032). Activation by Thr-186 phosphorylation is calcium Ca2+ signaling pathway-dependent; actively inactivated by dephosphorylation mediated by PPP1CA, PPM1A and PPM1B. Reversibly repressed by acetylation at Lys-44 and Lys-48.

Protein tissue specificity

Ubiquitous.

Involvement in disease

Note: Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy, and confers predisposition to heart failure.

Protein sequence and domain

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylation at Thr-186, Ser-347, Thr-350, Ser-353, Thr-354 and Ser-357 triggers kinase activity by promoting cyclin and substrate binding (e.g. HIV TAT) upon conformational changes. Thr-186 phosphorylation requires the calcium Ca2+ signaling pathway, including CaMK1D and calmodulin. This inhibition is relieved by Thr-29 dephosphorylation. However, phosphorylation at Thr-29 is inhibitory within the HIV transcription initiation complex. Phosphorylation at Ser-175 inhibits kinase activity. Can be phosphorylated on either Thr-362 or Thr-363 but not on both simultaneously . / Dephosphorylation of Thr-186 by PPM1A and PPM1B blocks CDK9 activity and may lead to CDK9 proteasomal degradation. However, PPP1CA-mediated Thr-186 dephosphorylation is required to release P-TEFb from its inactive P-TEFb/7SK snRNP complex. Dephosphorylation of C-terminus Thr and Ser residues by protein phosphatase-1 (PP1) triggers CDK9 activity, contributing to the activation of HIV-1 transcription. / N6-acetylation of Lys-44 by CBP/p300 promotes kinase activity, whereas acetylation of both Lys-44 and Lys-48 mediated by PCAF/KAT2B and GCN5/KAT2A reduces kinase activity. The acetylated form associates with PML bodies in the nuclear matrix and with the transcriptionally silent HIV-1 genome; deacetylated upon transcription stimulation. / Polyubiquitinated and thus activated by UBR5. This ubiquitination is promoted by TFIIS/TCEA1 and favors ‘Ser-2’ phosphorylation of RPB1/POLR2A CTD.

Protein cellular localization

Nucleus / Cytoplasm / Nucleus > PML body

Research area

All research areas>Cell Cycle Proteins>Cdk
(View all antibody categories related to Cell Cycle Proteins)

Note

AntibodyPlus can customize Cdk9 (phospho Thr186) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!