Rabbit Polyclonal Phospho-CDKN1A-T145 antibody (AP0327)

$99.00$259.00

Reactivity: Human
Applications: IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CDKN1A-T145 antibody (AP0327)

Supplier: ABclonal Inc.

Recommended applications: IHC

Recommended dilution:

IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

P21 antibody; CIP1 antibody; SDI1 antibody; WAF1 antibody; CAP20 antibody; CDKN1 antibody; MDA-6 antibody; p21CIP1 antibody;
CAP20 antibody|CDK-interacting protein 1 antibody|CDKI antibody|CDKN1 antibody|Cdkn1a antibody|CDN1A_HUMAN antibody|CIP1 antibody|Cyclin Dependent Kinase Inhibitor 1A antibody|Cyclin-dependent kinase inhibitor 1 antibody|Cyclin-dependent kinase inhibitor 1A (P21) antibody|Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody|DNA Synthesis Inhibitor antibody|MDA-6 antibody|MDA6 antibody|Melanoma differentiation-associated protein 6 antibody|Melanoma differentiation-associated protein antibody|p21 antibody|P21 protein antibody|p21CIP1 antibody|p21Cip1/Waf1 antibody|p21WAF antibody|PIC1 antibody|SDI1 antibody|SLC12A9 antibody|WAF1 antibody|Wild type p53 activated fragment 1 (WAF1) antibody|Wild type p53 activated fragment 1 antibody|Wildtype p53-activated fragment 1 antibody|Anti-p21 antibody [EPR362] (ab109520)

SCBT cat No: sc-71811|sc-817|sc-53393|sc-271532|sc-397|sc-56335|sc-6246|sc-271610|sc-756|sc-471|sc-469|sc-65595|sc-53870|sc-51689|sc-136020|sc-166630|sc-68396|sc-398612|sc-398750|

Name

Rabbit Polyclonal Phospho-CDKN1A-T145 antibody

Catalogue No.

AP0327

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T145 of human CDKN1A

Host

Rabbit

Applications

IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

21kDa

Formulation

Phospho-CDKN1A-T145 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

P21 antibody; CIP1 antibody; SDI1 antibody; WAF1 antibody; CAP20 antibody; CDKN1 antibody; MDA-6 antibody; p21CIP1 antibody;

Protein names

P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1;

Protein function

May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).

Protein tissue specificity

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Protein sequence and domain

Belongs to the CDI family. The PIP-box K+4 motif mediates both the interaction with PCNA and the recruitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.; The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.

Protein post-translational modifications

Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation.; Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114; leading to proteasomal degradation.; Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1 (By similarity).

Protein cellular localization

Cytoplasm. Nucleus.

Background

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Cell-Cycle-Proteins/">Cell Cycle Proteins</a>><a href="http://antibodyplus.com/product-tag/p21/">p21</a><br><a href="http://antibodyplus.com/product-tag/Cell-Cycle-Proteins/"&gt; (View all antibody categories related to Cell Cycle Proteins)</a>

Note

AntibodyPlus can customize Phospho-CDKN1A-T145 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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