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Rabbit Polyclonal Phospho-C/EBP alpha (T230) antibody (STJ90623)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for C/EBP alpha (phospho Thr230) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CEBPA antibody, CEBP antibody,|Apoptotic cysteine protease antibody|Apoptotic protease Mch-5 antibody|CAP4 antibody|CASP-8 antibody|CASP8 antibody|CASP8_HUMAN antibody|Caspase 8 antibody|Caspase-8 subunit p10 antibody|FADD-homologous ICE/CED-3-like protease antibody|FADD-like ICE antibody|FLICE antibody|ICE-like apoptotic protease 5 antibody|MACH antibody|MORT1-associated CED-3 homolog antibody|Anti-CEBP Alpha antibody [EP709Y] (ab40764)
SCBT cat No: sc-61|sc-9314|sc-365318|sc-166258|sc-9315|
C/EBP alpha (phospho Thr230) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-C/EBP alpha (T230) Polyclonal Antibody detects endogenous levels of C/EBP alpha protein only when phosphorylated at T230.
Synthesized phospho-peptide derived from C/EBP alpha (phospho Thr230) at AA range 170-250
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
C/EBP alpha (phospho Thr230) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
C/EBP alpha (phospho Thr230) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
CCAAT/enhancer-binding protein alpha antibody, C/EBP alpha antibody
|Protein names|| |
CCAAT/enhancer-binding protein alpha , C/EBP alpha
|Protein function|| |
Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5′-T[TG]NNGNAA[TG]-3′ acting as an activator on distinct target genes . During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex . In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity). / Isoform 3: Can act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation . / Isoform 4: Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.
|Involvement in disease|| |
Leukemia, acute myelogenous (AML) [MIM:601626]: A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
Belongs to the bZIP family. C/EBP subfamily. / Contains 1 bZIP (basic-leucine zipper) domain.
|Protein post-translational modifications|| |
Phosphorylation at Ser-190 is required for interaction with CDK2, CDK4 and SWI/SNF complex leading to cell cycle inhibiton. Dephosphorylated at Ser-190 by protein phosphatase 2A (PP2A) through PI3K/AKT signaling pathway regulation . Phosphorylation at Thr-226 and Thr-230 by GSK3 is constitutive in adipose tissue and lung. In liver, both Thr-226 and Thr-230 are phosphorylated only during feeding but not during fasting. Phosphorylation of the GSK3 consensus sites selectively decreases transactivation activity on IRE-controlled promoters. / Sumoylated, sumoylation blocks the inhibitory effect on cell proliferation by disrupting the interaction with SMARCA2.
|Protein cellular localization|| |
Nucleus / Nucleus > nucleolus
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St John’s Laboratory Ltd.
|Product type|| |
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