Rabbit Polyclonal Phospho-C/EBP alpha (T230) antibody (STJ90623)

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Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-C/EBP alpha (T230) antibody (STJ90623)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for C/EBP alpha (phospho Thr230) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CEBPA antibody, CEBP antibody,|Apoptotic cysteine protease antibody|Apoptotic protease Mch-5 antibody|CAP4 antibody|CASP-8 antibody|CASP8 antibody|CASP8_HUMAN antibody|Caspase 8 antibody|Caspase-8 subunit p10 antibody|FADD-homologous ICE/CED-3-like protease antibody|FADD-like ICE antibody|FLICE antibody|ICE-like apoptotic protease 5 antibody|MACH antibody|MORT1-associated CED-3 homolog antibody|Anti-CEBP Alpha antibody [EP709Y] (ab40764)
SCBT cat No: sc-61|sc-9314|sc-365318|sc-166258|sc-9315|

 

Name

C/EBP alpha (phospho Thr230) Polyclonal Antibody

Catalogue No.

STJ90623

Reactivity

Human, Mouse, Rat

Specificity

Phospho-C/EBP alpha (T230) Polyclonal Antibody detects endogenous levels of C/EBP alpha protein only when phosphorylated at T230.

Immunogen

Synthesized phospho-peptide derived from C/EBP alpha (phospho Thr230) at AA range 170-250

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

42 kDa

Formulation

C/EBP alpha (phospho Thr230) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

C/EBP alpha (phospho Thr230) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

CCAAT/enhancer-binding protein alpha antibody, C/EBP alpha antibody

Database links

Human UniProt/Swiss-Prot:P49715;Mouse UniPort/Swiss-Prot: P53566;Rat UniProt/Swiss-Port: P05554;Human Entrez Gene: 1050;Mouse Entrez Gene: 12606;

Protein names

CCAAT/enhancer-binding protein alpha , C/EBP alpha

Protein function

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5′-T[TG]NNGNAA[TG]-3′ acting as an activator on distinct target genes . During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex . In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity). / Isoform 3: Can act as dominant-negative. Binds DNA and have transctivation activity, even if much less efficiently than isoform 2. Does not inhibit cell proliferation . / Isoform 4: Directly and specifically enhances ribosomal DNA transcription interacting with RNA polymerase I-specific cofactors and inducing histone acetylation.

Involvement in disease

Leukemia, acute myelogenous (AML) [MIM:601626]: A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the bZIP family. C/EBP subfamily. / Contains 1 bZIP (basic-leucine zipper) domain.

Protein post-translational modifications

Phosphorylation at Ser-190 is required for interaction with CDK2, CDK4 and SWI/SNF complex leading to cell cycle inhibiton. Dephosphorylated at Ser-190 by protein phosphatase 2A (PP2A) through PI3K/AKT signaling pathway regulation . Phosphorylation at Thr-226 and Thr-230 by GSK3 is constitutive in adipose tissue and lung. In liver, both Thr-226 and Thr-230 are phosphorylated only during feeding but not during fasting. Phosphorylation of the GSK3 consensus sites selectively decreases transactivation activity on IRE-controlled promoters. / Sumoylated, sumoylation blocks the inhibitory effect on cell proliferation by disrupting the interaction with SMARCA2.

Protein cellular localization

Nucleus / Nucleus > nucleolus

Research area

All research areas>Transcription Regulators>C/EBP
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize C/EBP alpha (phospho Thr230) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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