Rabbit Polyclonal Phospho-C/EBP beta (T235) antibody (STJ90200)


Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-C/EBP beta (T235) antibody (STJ90200)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for C/EBP beta (phospho Thr235) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


CEBPB antibody, TCF5 antibody, PP9092 antibody,|AGP/EBP antibody|CCAAT Enhancer Binding Protein beta antibody|CCAAT/enhancer-binding protein beta antibody|CEBPB antibody|CEBPB_HUMAN antibody|CRP2 antibody|IL 6DBP antibody|IL6DBP antibody|Interleukin 6 dependent binding protein antibody|LAP antibody|Liver activator protein antibody|Liver enriched transcriptional activator antibody|NF IL6 antibody|NFIL6 antibody|Nuclear factor NF IL6 antibody|Nuclear factor NF-IL6 antibody|SF B antibody|SFB antibody|Silencer factor B antibody|TCF-5 antibody|TCF5 antibody|Transcription factor 5 antibody|Anti-CEBP Beta antibody [E299] (ab32358)
SCBT cat No: sc-16993|



C/EBP beta (phospho Thr235) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-C/EBP beta (T235) Polyclonal Antibody detects endogenous levels of C/EBP beta protein only when phosphorylated at T235.


Synthesized phospho-peptide derived from C/EBP beta (phospho Thr235) at AA range 180-260





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;







Molecular weight

36 kDa


C/EBP beta (phospho Thr235) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


C/EBP beta (phospho Thr235) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

CCAAT/enhancer-binding protein beta antibody, C/EBP beta antibody, Liver activator protein antibody, LAP antibody, Liver-enriched inhibitory protein antibody, LIP antibody, Nuclear factor NF-IL6 antibody, Transcription factor 5 antibody, TCF-5 antibody

Database links

Human UniProt/Swiss-Prot:P17676;Mouse UniPort/Swiss-Prot: P28033;Rat UniProt/Swiss-Port: P21272;Human Entrez Gene: 1051;Mouse Entrez Gene: 12608;Rat Entrez Gene: Rn.6479

Protein names

CCAAT/enhancer-binding protein beta , C/EBP beta , Liver activator protein , LAP , Liver-enriched inhibitory protein , LIP , Nuclear factor NF-IL6 , Transcription factor 5 , TCF-5

Protein function

Important transcription factor regulating the expression of genes involved in immune and inflammatory responses . Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5′-T[TG]NNGNAA[TG]-3′. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation . Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis (By similarity). / Isoform 2: Essential for gene expression induction in activated macrophages. Plays a major role in immune responses such as CD4+ T-cell response, granuloma formation and endotoxin shock. Not essential for intracellular bacteria killing. / Isoform 3: Acts as a dominant negative through heterodimerization with isoform 2 . Promotes osteoblast differentiation and osteoclastogenesis (By similarity).

Protein tissue specificity

Expressed at low levels in the lung, kidney and spleen.

Protein sequence and domain

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. / Belongs to the bZIP family. C/EBP subfamily. / Contains 1 bZIP (basic-leucine zipper) domain.

Protein post-translational modifications

Methylated. Methylation at Arg-3 by CARM1 and at Lys-43 by EHMT2 inhibit transactivation activity. Methylation is probably inhibited by phosphorylation at Thr-235. / Sumoylated by polymeric chains of SUMO2 or SUMO3 . Sumoylation at Lys-174 is required for inhibition of T-cells proliferation. In adipocytes, sumoylation at Lys-174 by PIAS1 leads to ubiquitination and subsequent proteasomal degradation. Desumoylated by SENP2, which abolishes ubiquitination and stabilizes protein levels (By similarity). / Ubiquitinated, leading to proteasomal degradation. / Phosphorylated at Thr-235 by MAPK and CDK2, serves to prime phosphorylation at Thr-226 and Ser-231 by GSK3B and acquire DNA-binding as well as transactivation activities, required to induce adipogenesis. MAPK and CDK2 act sequentially to maintain Thr-235 in the primed phosphorylated state during mitotical cloning expansion and thereby progression of terminal differentiation. Phosphorylation at Thr-266 enhances transactivation activity. Phosphorylation at Ser-325 in response to calcium increases transactivation activity. Phosphorylated at Thr-235 by RPS6KA1 . / O-glycosylated, glycosylation at Ser-227 and Ser-228 prevents phosphorylation on Thr-235, Ser-231 and Thr-226 and DNA binding activity which delays the adipocyte differentiation program. / Acetylated. Acetylation at Lys-43 is an important and dynamic regulatory event that contributes to its ability to transactivate target genes, including those associated with adipogenesis and adipocyte function. Deacetylation by HDAC1 represses its transactivation activity. Acetylated by KAT2A and KAT2B within a cluster of lysine residues between amino acids 129-133, this acetylation is strongly induced by glucocorticoid treatment and enhances transactivation activity.

Protein cellular localization

Nucleus / Cytoplasm

Research area

All research areas>Transcription Regulators>C/EBP
(View all antibody categories related to Transcription Regulators)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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