Rabbit Polyclonal Phospho-CFL1-Y139 antibody (AP0330)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CFL1-Y139 antibody (AP0330)

Supplier: ABclonal Inc.

Recommended applications: IHC

Recommended dilution:

IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-CFL1-Y139 antibody.

Alternative names:

Check alternative names for the antibody

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CFL antibody;
18 kDa phosphoprotein antibody|CFL antibody|Cofilin 1 (non-muscle) antibody|cofilin 1 antibody|Cofilin 2 (muscle) antibody|cofilin 2 antibody|Cofilin, muscle isoform antibody|Cofilin, non-muscle isoform antibody|NEM7 antibody|p18 antibody|Anti-Cofilin antibody (ab42824)

SCBT cat No: sc-53934|sc-32158|sc-32157|sc-166958|sc-32160|sc-376476|sc-33779|sc-8441|sc-136179|sc-164079|sc-130444|

Name

Rabbit Polyclonal Phospho-CFL1-Y139 antibody

Catalogue No.

AP0330

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding Y139 of human CFL1

Host

Rabbit

Applications

IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

19kDa

Formulation

Phospho-CFL1-Y139 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CFL antibody;

Protein names

CFL;

Protein function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

Protein tissue specificity

Widely distributed in various tissues.

Protein sequence and domain

Belongs to the actin-binding proteins ADF family.; Contains 1 ADF-H domain.

Protein post-translational modifications

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.

Protein cellular localization

Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Structural-Proteins/">Structural Proteins</a>><a href="http://antibodyplus.com/product-tag/Cofilin/">Cofilin</a><br><a href="http://antibodyplus.com/product-tag/Structural-Proteins/"&gt; (View all antibody categories related to Structural Proteins)</a>

Note

AntibodyPlus can customize Phospho-CFL1-Y139 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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