Rabbit Polyclonal Phospho-CFL1-Y88/CFL2-Y88 antibody (AP0329)

$99.00$259.00

Reactivity: Human,Mouse
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CFL1-Y88/CFL2-Y88 antibody (AP0329)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-CFL1-Y88/CFL2-Y88 antibody.

Alternative names:

Check alternative names for the antibody

Expand

CFL1/CFL2 antibody;
18 kDa phosphoprotein antibody|CFL antibody|Cofilin 1 (non-muscle) antibody|cofilin 1 antibody|Cofilin 2 (muscle) antibody|cofilin 2 antibody|Cofilin, muscle isoform antibody|Cofilin, non-muscle isoform antibody|NEM7 antibody|p18 antibody|Anti-Cofilin antibody (ab42824)

SCBT cat No: sc-53934|sc-32158|sc-32157|sc-166958|sc-32160|sc-376476|sc-33779|sc-8441|sc-136179|sc-164079|sc-130444|

Name

Rabbit Polyclonal Phospho-CFL1-Y88/CFL2-Y88 antibody

Catalogue No.

AP0329

Reactivity

Human, Mouse

Immunogen

A phospho specific peptide corresponding to residues surrounding Y88 of human CFL1/CFL2

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

19kDa

Formulation

Phospho-CFL1-Y88/CFL2-Y88 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CFL1/CFL2 antibody;

Protein names

CFL1/CFL2;

Protein function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:11812157, PubMed:15580268, PubMed:21834987, PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

Protein tissue specificity

Widely distributed in various tissues.

Protein sequence and domain

Belongs to the actin-binding proteins ADF family.; Contains 1 ADF-H domain.

Protein post-translational modifications

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.

Protein cellular localization

Nucleus matrix . Cytoplasm, cytoskeleton . Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, lamellipodium membrane ; Peripheral membrane protein ; Cytoplasmic side . Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide.

Background

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Structural-Proteins/">Structural Proteins</a>><a href="http://antibodyplus.com/product-tag/Cofilin/">Cofilin</a><br><a href="http://antibodyplus.com/product-tag/Structural-Proteins/"&gt; (View all antibody categories related to Structural Proteins)</a>

Note

AntibodyPlus can customize Phospho-CFL1-Y88/CFL2-Y88 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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