Rabbit Polyclonal Phospho-CHEK2-S33/35 antibody (AP0545)

$99.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CHEK2-S33/35 antibody (AP0545)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

CDS1 antibody; CHK2 antibody; LFS2 antibody; RAD53 antibody; hCds1 antibody; HuCds1 antibody; PP1425 antibody;
CDS 1 antibody|Cds1 antibody|Cds1 homolog antibody|Checkpoint kinase 2 antibody|Checkpoint like protein CHK2 antibody|CHEK 2 antibody|Chek2 antibody|Chk 2 antibody|CHK2 checkpoint homolog (S. pombe) antibody|CHK2 checkpoint homolog antibody|CHK2_HUMAN antibody|hCds1 antibody|HuCds 1 antibody|LFS 2 antibody|LFS2 antibody|PP1425 antibody|RAD 53 antibody|RAD53 antibody|Rad53 homolog antibody|Serine/threonine protein kinase Chk2 antibody|Serine/threonine-protein kinase Chk2 antibody|Anti-Chk2 antibody [EPR4325] (ab109413) KO Validated

SCBT cat No: sc-136251|sc-17747|sc-5278|sc-17748|sc-56296|sc-56297|sc-9064|sc-8812|sc-293293|sc-34986|

Name

Rabbit Polyclonal Phospho-CHEK2-S33/35 antibody

Catalogue No.

AP0545

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S33/35 of human CHEK2

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

61kDa

Formulation

Phospho-CHEK2-S33/35 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CDS1 antibody; CHK2 antibody; LFS2 antibody; RAD53 antibody; hCds1 antibody; HuCds1 antibody; PP1425 antibody;

Protein names

CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425;

Protein function

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

Protein tissue specificity

High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.; Contains 1 FHA domain.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4.; Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination.

Protein cellular localization

Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell.; Isoform 4: Nucleus.; Isoform 7: Nucleus.; Isoform 9: Nucleus.; Isoform 12: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Note=Recruited into PML bodies together with TP53.

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Cell-Cycle-Proteins/">Cell Cycle Proteins</a>><a href="http://antibodyplus.com/product-tag/Chk/">Chk</a><br><a href="http://antibodyplus.com/product-tag/Cell-Cycle-Proteins/"&gt; (View all antibody categories related to Cell Cycle Proteins)</a>

Note

AntibodyPlus can customize Phospho-CHEK2-S33/35 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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