Rabbit Polyclonal Phospho-CHUK-T23 antibody (AP0173)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CHUK-T23 antibody (AP0173)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-CHUK-T23 antibody.

Alternative names:

Check alternative names for the antibody

Expand

IKK1 antibody; IKKA antibody; IKBKA antibody; TCF16 antibody; NFKBIKA antibody; IKK-alpha antibody;
chuk antibody|CHUK1 antibody|Conserved Helix Loop Helix Ubiquitous Kinase antibody|Conserved helix loop ubiquitous kinase antibody|Conserved helix-loop-helix ubiquitous kinase antibody|I Kappa B Kinase 1 antibody|I Kappa B Kinase Alpha antibody|I-kappa-B kinase 1 antibody|I-kappa-B kinase alpha antibody|IkappaB kinase antibody|IkB kinase alpha subunit antibody|IkBKA antibody|IKK 1 antibody|IKK A antibody|IKK a kinase antibody|IKK-A antibody|IKK-alpha antibody|IKK1 antibody|IKKA antibody|IKKA_HUMAN antibody|Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody|Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody|NFKBIKA antibody|Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody|Nuclear factor NF kappa B inhibitor kinase alpha antibody|Nuclear factor NF-kappa-B inhibitor kinase alpha antibody|Nuclear factor NFkappaB inhibitor kinase alpha antibody|Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody|TCF-16 antibody|TCF16 antibody|Transcription factor 16 antibody|Anti-IKK alpha antibody [Y463] (ab32041)

SCBT cat No: sc-7190|

Name

Rabbit Polyclonal Phospho-CHUK-T23 antibody

Catalogue No.

AP0173

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding T23 of human CHUK

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

85kDa

Formulation

Phospho-CHUK-T23 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

IKK1 antibody; IKKA antibody; IKBKA antibody; TCF16 antibody; NFKBIKA antibody; IKK-alpha antibody;

Protein names

IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha;

Protein function

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor (PubMed:15084260).

Protein tissue specificity

Widely expressed.

Protein sequence and domain

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.; Contains 1 protein kinase domain. The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG.

Protein post-translational modifications

Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.; (Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.

Protein cellular localization

Cytoplasm . Nucleus . Note=Shuttles between the cytoplasm and the nucleus.

Background

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/IKK-alpha/">IKK alpha</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-CHUK-T23 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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