Rabbit Polyclonal Phospho-Cofilin (S3) antibody (STJ90230)

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Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Cofilin (S3) antibody (STJ90230)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cofilin (phospho Ser3) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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CFL1 antibody, CFL antibody,|18 kDa phosphoprotein antibody|CFL antibody|Cofilin 1 (non-muscle) antibody|cofilin 1 antibody|Cofilin 2 (muscle) antibody|cofilin 2 antibody|Cofilin, muscle isoform antibody|Cofilin, non-muscle isoform antibody|NEM7 antibody|p18 antibody|Anti-Cofilin antibody (ab42824)
SCBT cat No: sc-53934|sc-32158|sc-32157|sc-166958|sc-32160|sc-376476|sc-33779|sc-8441|sc-136179|sc-164079|sc-130444|

 

Name

Cofilin (phospho Ser3) Polyclonal Antibody

Catalogue No.

STJ90230

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Cofilin (S3) Polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at S3.

Immunogen

Synthesized phospho-peptide derived from Cofilin (phospho Ser3) at AA range 10-90

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

19 kDa

Formulation

Cofilin (phospho Ser3) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cofilin (phospho Ser3) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Cofilin-1 antibody, 18 kDa phosphoprotein antibody, p18 antibody, Cofilin, non-muscle isoform antibody

Database links

Human UniProt/Swiss-Prot:P23528;Mouse UniPort/Swiss-Prot: P18760;Rat UniProt/Swiss-Port: P45592;Human Entrez Gene: 3716;Mouse Entrez Gene: 12631;Rat Entrez Gene: Rn.11675

Protein names

Cofilin-1 , 18 kDa phosphoprotein , p18 , Cofilin, non-muscle isoform

Protein function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

Protein tissue specificity

Widely distributed in various tissues.

Protein sequence and domain

Belongs to the actin-binding proteins ADF family. / Contains 1 ADF-H domain.

Protein post-translational modifications

Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells (By similarity). Dephosphorylated by PDXP/chronophin; this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal (By similarity). Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2.

Protein cellular localization

Nucleus matrix / Cytoplasm > cytoskeleton / Cell projection > ruffle membrane / Peripheral membrane protein / Cytoplasmic side / Cell projection > lamellipodium membrane / Peripheral membrane protein / Cytoplasmic side

Research area

All research areas>Structural Proteins>Cofilin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize Cofilin (phospho Ser3) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 193

Validation data ID: 193

Submission date: 2017-05-21

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Result figure:
Result figure

Figure description:

we used this antibody to detect p-cofilin in HEK293T cells and could detect a band around 18-19 KD.

Product name

anti-P-cofilin

Product catalog No.

STJ90230

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Excellent

Antibody overall rating

Excellent

Your comments

It is a good antibody for western blotting

Detailed protocol

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