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Rabbit Polyclonal Phospho-Cot (T290) antibody (STJ91027)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for Cot (phospho Thr290) Polyclonal Antibody.
Check alternative names for the antibodyExpand
MAP3K8 antibody, COT antibody, ESTF antibody,|AURA2 antibody|c COT antibody|Cancer Osaka thyroid oncogene antibody|CCOT antibody|COT antibody|COT proto oncogene serine/threonine protein kinase antibody|EST antibody|ESTF antibody|Ewing sarcoma transformant antibody|FLJ10486 antibody|M3K8_HUMAN antibody|MAP3K 8 antibody|MAP3K8 antibody|MEKK8 antibody|Mitogen activated protein kinase kinase kinase 8 antibody|Mitogen-activated protein kinase kinase kinase 8 antibody|Proto oncogene cCot antibody|Proto-oncogene c-Cot antibody|Serine/threonine protein kinase cot antibody|Serine/threonine-protein kinase cot antibody|TPL 2 antibody|TPL-2 antibody|TPL2 antibody|Tumor progression locus 2 antibody|Anti-MAP3K8 antibody (ab137589)
SCBT cat No: sc-9167|sc-373677|sc-720|sc-1717|
Cot (phospho Thr290) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Cot (T290) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at T290.
Synthesized phospho-peptide derived from Cot (phospho Thr290) at AA range 230-310
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;
|Molecular weight|| |
Cot (phospho Thr290) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Cot (phospho Thr290) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Mitogen-activated protein kinase kinase kinase 8 antibody, Cancer Osaka thyroid oncogene antibody, Proto-oncogene c-Cot antibody, Serine/threonine-protein kinase cot antibody, Tumor progression locus 2 antibody, TPL-2 antibody
|Protein names|| |
Mitogen-activated protein kinase kinase kinase 8 , Cancer Osaka thyroid oncogene , Proto-oncogene c-Cot , Serine/threonine-protein kinase cot , Tumor progression locus 2 , TPL-2
|Protein function|| |
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. / ATP + a protein = ADP + a phosphoprotein. / Mg2+
|Protein tissue specificity|| |
Expressed in several normal tissues and human tumor-derived cell lines.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Thr-290 is autophosphorylated and/or transphosphorylated ; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Ser-400 is autophosphorylated and/or transphosphorylated by IKBKB ; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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