Rabbit Polyclonal Phospho-Cot (T290) antibody (STJ91027)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-Cot (T290) antibody (STJ91027)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Cot (phospho Thr290) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

Expand

MAP3K8 antibody, COT antibody, ESTF antibody,|AURA2 antibody|c COT antibody|Cancer Osaka thyroid oncogene antibody|CCOT antibody|COT antibody|COT proto oncogene serine/threonine protein kinase antibody|EST antibody|ESTF antibody|Ewing sarcoma transformant antibody|FLJ10486 antibody|M3K8_HUMAN antibody|MAP3K 8 antibody|MAP3K8 antibody|MEKK8 antibody|Mitogen activated protein kinase kinase kinase 8 antibody|Mitogen-activated protein kinase kinase kinase 8 antibody|Proto oncogene cCot antibody|Proto-oncogene c-Cot antibody|Serine/threonine protein kinase cot antibody|Serine/threonine-protein kinase cot antibody|TPL 2 antibody|TPL-2 antibody|TPL2 antibody|Tumor progression locus 2 antibody|Anti-MAP3K8 antibody (ab137589)
SCBT cat No: sc-9167|sc-373677|sc-720|sc-1717|

 

Name

Cot (phospho Thr290) Polyclonal Antibody

Catalogue No.

STJ91027

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Cot (T290) Polyclonal Antibody detects endogenous levels of Cot protein only when phosphorylated at T290.

Immunogen

Synthesized phospho-peptide derived from Cot (phospho Thr290) at AA range 230-310

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

60 kDa

Formulation

Cot (phospho Thr290) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Cot (phospho Thr290) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Mitogen-activated protein kinase kinase kinase 8 antibody, Cancer Osaka thyroid oncogene antibody, Proto-oncogene c-Cot antibody, Serine/threonine-protein kinase cot antibody, Tumor progression locus 2 antibody, TPL-2 antibody

Database links

Human UniProt/Swiss-Prot:P41279;Mouse UniPort/Swiss-Prot: Q07174;Rat UniProt/Swiss-Port: Q63562;Human Entrez Gene: 1326;Mouse Entrez Gene: 26410;Rat Entrez Gene: Rn.9939

Protein names

Mitogen-activated protein kinase kinase kinase 8 , Cancer Osaka thyroid oncogene , Proto-oncogene c-Cot , Serine/threonine-protein kinase cot , Tumor progression locus 2 , TPL-2

Protein function

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant. / ATP + a protein = ADP + a phosphoprotein. / Mg2+

Protein tissue specificity

Expressed in several normal tissues and human tumor-derived cell lines.

Protein sequence and domain

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues. Thr-290 is autophosphorylated and/or transphosphorylated ; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation. Ser-400 is autophosphorylated and/or transphosphorylated by IKBKB ; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages.

Protein cellular localization

Cytoplasm

Research area

All research areas>Kinases and Phosphatases>Cot
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize Cot (phospho Thr290) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:



Welcome to AntibodyPlus!