Rabbit Polyclonal Phospho-CREB1-S133 antibody (AP0333)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CREB1-S133 antibody (AP0333)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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CREB-1 antibody; CREB1 antibody;
Active transcription factor CREB antibody|Extracellular signal-regulated kinase 2 antibody|HS44KDAP antibody|HUMKER1A antibody|Insulin stimulated MAP2 kinase antibody|MAP kinase 1 antibody|MAP kinase 2 antibody|MAP kinase 3 antibody|MAP kinase isoform p42 antibody|MAP kinase isoform p44 antibody|MAPK 1 antibody|MAPK 2 antibody|Mapk1 antibody|MAPK2 antibody|MAPK3 antibody|Microtubule associated protein 2 kinase antibody|Mitogen activated protein kinase 3 antibody|Mitogen-activated protein kinase 1 antibody|Mitogen-activated protein kinase 2 antibody|MK01_HUMAN antibody|p38 antibody|p40 antibody|p41 antibody|p41mapk antibody|p42-MAPK antibody|P42MAPK antibody|p44 ERK1 antibody|p44 MAPK antibody|P44ERK1 antibody|P44MAPK antibody|PRKM1 antibody|PRKM2 antibody|PRKM3 antibody|Protein tyrosine kinase ERK2 antibody|Transactivator protein antibody|Phospho anti-CREB (S133) antibody [E113] (ab32096)

SCBT cat No: sc-58|sc-271|sc-186|sc-377154|sc-374227|sc-25785|sc-240|sc-390063|sc-7583|

Name

Rabbit Polyclonal Phospho-CREB1-S133 antibody

Catalogue No.

AP0333

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S133 of human CREB1

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

43kDa

Formulation

Phospho-CREB1-S133 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CREB-1 antibody; CREB1 antibody;

Protein names

CREB-1; CREB1;

Protein function

Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Protein sequence and domain

Belongs to the bZIP family.; Contains 1 bZIP (basic-leucine zipper) domain.; Contains 1 KID (kinase-inducible) domain.

Protein post-translational modifications

Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. In liver, phosphorylation is induced by fasting or glucagon in a circadian fashion (By similarity). CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity (By similarity). Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1 (By similarity). Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.; Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.

Protein cellular localization

Nucleus .

Background

CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/Akt/">Akt</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-CREB1-S133 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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