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Rabbit Polyclonal Phospho-Crk II (Y221) antibody (STJ90687)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for Crk II (phospho Tyr221) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CRK antibody,|Adapter molecule crk antibody|Avian sarcoma virus CT10 (v crk) oncogene homolog antibody|CRK antibody|CRK isoform 2 antibody|CRK isoform II antibody|CRK_HUMAN antibody|CRKII antibody|FLJ38130 antibody|OTTHUMP00000115366 antibody|OTTHUMP00000198330 antibody|p38 antibody|Proto oncogene C crk antibody|Proto-oncogene C-crk antibody|v crk avian sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog antibody|v crk sarcoma virus CT10 oncogene homolog (avian) antibody|Anti-Crk p38 antibody [EPR4984(2)] (ab133581)
SCBT cat No: sc-393160|sc-23775|sc-17989|sc-81234|sc-390132|sc-289|sc-9004|
Crk II (phospho Tyr221) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat, Monkey
Phospho-Crk II (Y221) Polyclonal Antibody detects endogenous levels of Crk II protein only when phosphorylated at Y221.
Synthesized phospho-peptide derived from Crk II (phospho Tyr221) at AA range 160-240
WB, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:40000;
|Molecular weight|| |
Crk II (phospho Tyr221) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Crk II (phospho Tyr221) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Adapter molecule crk antibody, Proto-oncogene c-Crk antibody, p38 antibody
|Protein names|| |
Adapter molecule crk , Proto-oncogene c-Crk , p38
|Protein function|| |
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
|Protein sequence and domain|| |
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation. / The SH2 domain mediates interaction with tyrosine phosphorylated proteins. Mediates interaction with SHB. / Belongs to the CRK family. / Contains 1 SH2 domain. / Contains 2 SH3 domains.
|Protein post-translational modifications|| |
Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. Isoform Crk-II is phosphorylated by KIT. / Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. / Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form.
|Protein cellular localization|| |
Cytoplasm / Cell membrane
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St John’s Laboratory Ltd.
|Product type|| |
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