Rabbit Polyclonal Phospho-CTTN-Y466 antibody (AP0247)

$99.00$259.00

Reactivity: Human,Mouse
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-CTTN-Y466 antibody (AP0247)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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EMS1 antibody;
Amplaxin antibody|CTTN antibody|EMS 1 antibody|EMS1 antibody|FLJ34459 antibody|Mammary tumor and squamous cell carcinoma associated antibody|Oncogene EMS1 antibody|p80/85 src substrate antibody|Src substrate cortactin antibody|SRC8_HUMAN antibody|Anti-Cortactin antibody [EP1922Y] (ab81208)

SCBT cat No: sc-136134|sc-55578|sc-55588|sc-30770|sc-30771|sc-6544|sc-271173|sc-11408|sc-55579|sc-30772|sc-242504|

Name

Rabbit Polyclonal Phospho-CTTN-Y466 antibody

Catalogue No.

AP0247

Reactivity

Human, Mouse

Immunogen

A phospho specific peptide corresponding to residues surrounding Y466 of human CTTN

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

85kDa

Formulation

Phospho-CTTN-Y466 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

EMS1 antibody;

Protein names

EMS1;

Protein function

Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454).

Protein sequence and domain

Contains 7 cortactin repeats.; Contains 1 SH3 domain. The SH3 motif may mediate binding to the cytoskeleton.

Protein post-translational modifications

Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associates with filamentous actin (By similarity). Phosphorylated on tyrosine residues in response to CHRM1 activation (By similarity). Phosphorylated by PTK2/FAK1 in response to cell adhesion (By similarity). Phosphorylated by FER. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding.

Protein cellular localization

Cytoplasm, cytoskeleton . Cell projection, lamellipodium . Cell projection, ruffle. Cell projection, dendrite . Cell projection . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, podosome . Cell junction . Cell junction, focal adhesion . Membrane, clathrin-coated pit . Cell projection, dendritic spine . Cytoplasm, cell cortex . Note=Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity).

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Signaling-Intermediates/">Signaling Intermediates</a>><a href="http://antibodyplus.com/product-tag/Cortactin/">Cortactin</a><br><a href="http://antibodyplus.com/product-tag/Signaling-Intermediates/"&gt; (View all antibody categories related to Signaling Intermediates)</a>

Note

AntibodyPlus can customize Phospho-CTTN-Y466 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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