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Rabbit Polyclonal Phospho-Cyclin B1 (S147) antibody (STJ90851)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for Cyclin B1 (phospho Ser147) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CCNB1 antibody, CCNB antibody,|CCNB 1 antibody|CCNB antibody|ccnb1 antibody|CCNB1_HUMAN antibody|Cyclin B1 antibody|G2 mitotic specific cyclin B1 antibody|G2/mitotic-specific cyclin-B1 antibody|Anti-Cyclin B1 antibody [V152] (ab72)
SCBT cat No: sc-245|
Cyclin B1 (phospho Ser147) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Cyclin B1 (S147) Polyclonal Antibody detects endogenous levels of Cyclin B1 protein only when phosphorylated at S147.
Synthesized phospho-peptide derived from Cyclin B1 (phospho Ser147) at AA range 90-170
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
|Molecular weight|| |
Cyclin B1 (phospho Ser147) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Cyclin B1 (phospho Ser147) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
G2/mitotic-specific cyclin-B1 antibody
|Protein names|| |
|Protein function|| |
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
|Protein tissue specificity|| |
Accumulates steadily during G2 and is abruptly destroyed at mitosis.
|Protein sequence and domain|| |
Belongs to the cyclin family. Cyclin AB subfamily.
|Protein post-translational modifications|| |
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. / Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Cytoplasm > cytoskeleton > microtubule organizing center > centrosome
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St John’s Laboratory Ltd.
|Product type|| |
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