Rabbit Polyclonal Phospho-DAPK3 (T265) antibody (STJ90708)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-DAPK3 (T265) antibody (STJ90708)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for DAPK3 (phospho Thr265) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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DAPK3 antibody, ZIPK antibody,|DAP kinase 3 antibody|DAP like kinase antibody|DAP-like kinase antibody|Dapk 3 antibody|DAPK3 antibody|DAPK3_HUMAN antibody|Death associated kinase 3 antibody|Death associated protein kinase 3 antibody|Death-associated protein kinase 3 antibody|Dlk antibody|EC 2.7.11.1 antibody|FLJ36473 antibody|MYPT1 kinase antibody|ZIP antibody|ZIP kinase antibody|ZIP kinase isoform antibody|ZIP-kinase antibody|ZIPK antibody|zipper-interacting protein kinase antibody|Anti-ZIP Kinase antibody [EPR18809-86] (ab210528)
SCBT cat No: To be updated

 

Name

DAPK3 (phospho Thr265) Polyclonal Antibody

Catalogue No.

STJ90708

Reactivity

Human, Mouse, Rat

Specificity

Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.

Immunogen

Synthesized phospho-peptide derived from DAPK3 (phospho Thr265) at AA range 210-290

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

52 kDa

Formulation

DAPK3 (phospho Thr265) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

DAPK3 (phospho Thr265) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Death-associated protein kinase 3 antibody, DAP kinase 3 antibody, DAP-like kinase antibody, Dlk antibody, MYPT1 kinase antibody, Zipper-interacting protein kinase antibody, ZIP-kinase antibody

Database links

Human UniProt/Swiss-Prot:O43293;Mouse UniPort/Swiss-Prot: O54784;Rat UniProt/Swiss-Port: O88764;Human Entrez Gene: 1613;Mouse Entrez Gene: 13144;Rat Entrez Gene: Rn.60353

Protein names

Death-associated protein kinase 3 , DAP kinase 3 , DAP-like kinase , Dlk , MYPT1 kinase , Zipper-interacting protein kinase , ZIP-kinase

Protein function

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca2+ and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on ‘Ser-77’ upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / A sequential activation is proposed: autophosphorylation at consensus sites is leading to dimerization of the catalytic domain stabilized by phosphorylation at Ser-50 and activation segment exchange (producing an active confirmation of both kinase modules in trans) followed by phosphorylation at Thr-180 in the activation segment and at other regulatory sites (Probable). Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. / 12 µM for myosin (isoform 2) / 6.2 µM for myosin (isoform 1) / 73 µM for MYL12B (isoform 2) / 10.4 µM for MYL12B (isoform 1) / 248 nmol/min/mg enzyme toward myosin (isoform 2) / 120 nmol/min/mg enzyme toward myosin (isoform 1) / 1.3 µmol/min/mg enzyme toward MYL12B (isoform 2) / 271 nmol/min/mg enzyme toward MYL12B (isoform 1)

Protein tissue specificity

Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.

Protein sequence and domain

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. / Contains 1 protein kinase domain.

Protein post-translational modifications

The phosphorylation status is critical for kinase activity, oligomerization and intracellular localization. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. The phosphorylated form is localized in the cytoplasm promoted by phosphorylation at Thr-299; nuclear translocation or retention is maximal when it is not phosphorylated. Phosphorylation increases the trimeric form, and its dephosphorylation favors a kinase-inactive monomeric form. Both isoform 1 and isoform 2 can undergo autophosphorylation.

Protein cellular localization

Nucleus / Cytoplasm / Nucleus / Cytoplasm / Nucleus / Cytoplasm

Research area

All research areas>Kinases and Phosphatases>ZIP-kinase
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize DAPK3 (phospho Thr265) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 14

Validation data ID: 14

Submission date: 2016-11-23

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Result figure:
Result figure

Figure description:

Signal seen in mouse photoreceptor inner segment, none or very week at nucleus.

Result figure:
blank.pdf

Figure description:

no western tested

Product name

Rabbit anti-Phospho-DAPK3

Product catalog No.

STJ90708

Product type

Primary antibody

Supplier

St John's Laboratory Ltd

Application type

Immunofluorescence (IF)

Antibody specificity rating

Poor

Antibody overall rating

Poor

Your comments

week signal in photoreceptor inner segment

Detailed protocol

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