Rabbit Polyclonal Phospho-eEF2K (S366) antibody (STJ90759)


Reactivity: Human, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-eEF2K (S366) antibody (STJ90759)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for eEF2K (phospho Ser366) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


EEF2K antibody,|C86191 antibody|Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody|Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase antibody|Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody|Calmodulin dependent protein kinase III antibody|cb365 antibody|EC antibody|eEF 2 kinase antibody|eEF 2K antibody|eEF-2 kinase antibody|eEF-2K antibody|Eef2k antibody|EEF2K protein antibody|EF2K_HUMAN antibody|Elongation factor 2 kinase antibody|Eukaryotic elongation factor 2 kinase antibody|fa04b08 antibody|HSU93850 antibody|kinase eEF2K antibody|MGC45041 antibody|SMEF2K antibody|wu:fa04b08 antibody|zgc:100973 antibody|Anti-EEF2K antibody [EP881Y] (ab45168)
SCBT cat No: sc-393366|sc-390710|sc-21642|sc-21641|sc-514961|sc-398028|sc-398019|sc-21758|sc-377439|sc-68481|sc-517178|



eEF2K (phospho Ser366) Polyclonal Antibody

Catalogue No.



Human, Rat


Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.


Synthesized phospho-peptide derived from eEF2K (phospho Ser366) at AA range 300-380





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;







Molecular weight

95 kDa


eEF2K (phospho Ser366) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


eEF2K (phospho Ser366) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Eukaryotic elongation factor 2 kinase antibody, eEF-2 kinase antibody, eEF-2K antibody, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody

Database links

Human UniProt/Swiss-Prot:O00418;Mouse UniPort/Swiss-Prot: O08796;Rat UniProt/Swiss-Port: P70531;Human Entrez Gene: 29904;Mouse Entrez Gene: 13631;Rat Entrez Gene: Rn.10958

Protein names

Eukaryotic elongation factor 2 kinase , eEF-2 kinase , eEF-2K , Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

Protein function

Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. / ATP + [elongation factor 2] = ADP + [elongation factor 2] phosphate. / Undergoes calcium/calmodulin-dependent intramolecular autophosphorylation, and this results in it becoming partially calcium/calmodulin-independent.

Protein sequence and domain

The catalytic domain is located to N-terminal region. The neighbor region contains the calmodulin-binding domain. / Belongs to the protein kinase superfamily. Alpha-type protein kinase family. / Contains 1 alpha-type protein kinase domain.

Protein post-translational modifications

Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.


AntibodyPlus can customize eEF2K (phospho Ser366) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


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