Rabbit polyclonal Phospho-EGFR-T669 antibody (AP0025)

$69.00$259.00

Reactivity: Human
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-EGFR-T669 antibody (AP0025)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-EGFR-T669 antibody.

Alternative names:

Check alternative names for the antibody

Expand

ERBB antibody, HER1 antibody, mENA antibody, ERBB1 antibody, PIG61 antibody
Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody|Cell growth inhibiting protein 40 antibody|Cell proliferation inducing protein 61 antibody|EGF R antibody|EGFR antibody|EGFR_HUMAN antibody|Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody|Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody|Epidermal growth factor receptor antibody|erb-b2 receptor tyrosine kinase 1 antibody|ERBB antibody|ERBB1 antibody|Errp antibody|HER1 antibody|mENA antibody|NISBD2 antibody|Oncogen ERBB antibody|PIG61 antibody|Proto-oncogene c-ErbB-1 antibody|Receptor tyrosine protein kinase ErbB 1 antibody|Receptor tyrosine-protein kinase ErbB-1 antibody|SA7 antibody|Species antigen 7 antibody|Urogastrone antibody|v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody|wa2 antibody|Wa5 antibody|Anti-EGFR antibody [EP38Y] (ab52894)

SCBT cat No: sc-373746|

Name

Rabbit polyclonal Phospho-EGFR-T669 antibody

Catalogue No.

AP0025

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T669 of human EGFR

Host

Rabbit

Applications

WB

Recommended dilution

WB 1:500 – 1:2000

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: 134kDa/Observed: Refer to Figures

Formulation

Phospho-EGFR-T669 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-EGFR-T669 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

ERBB antibody, HER1 antibody, mENA antibody, ERBB1 antibody, PIG61 antibody

Database links

Human UniProt/Swiss-Prot:P00533

Protein names

ERBB, HER1, mENA, ERBB1, PIG61

Protein function

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.; Isoform 2 may act as an antagonist of EGF action.

Protein tissue specificity

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.

Protein sequence and domain

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.; Contains 1 protein kinase domain.

Protein post-translational modifications

Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.; Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through ‘Lys-63’, but linkage through ‘Lys-48’, ‘Lys-11’ and ‘Lys-29’ also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).; Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.

Protein cellular localization

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. Note: In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).; Isoform 2: Secreted.

Background

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Research area

All research areas>Membrane Receptors>EGFR
(View all antibody categories related to Membrane Receptors)

Note

AntibodyPlus can customize Phospho-EGFR-T669 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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