Rabbit Polyclonal Phospho-EIF2AK2-T451 antibody (AP0340)

$99.00$259.00

Reactivity: Human
Applications: IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-EIF2AK2-T451 antibody (AP0340)

Supplier: ABclonal Inc.

Recommended applications: IHC

Recommended dilution:

IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

PKR antibody; PRKR antibody; EIF2AK1 antibody;
Double stranded RNA activated protein kinase; antibody|E2AK2_HUMAN antibody|eIF-2A protein kinase 2 antibody|EIF2AK1 antibody|EIF2AK2 antibody|Eukaryotic translation initiation factor 2 alpha kinase 2 antibody|Eukaryotic translation initiation factor 2-alpha kinase 2 antibody|HGNC:9437 antibody|Interferon induced double stranded RNA activated protein kinase antibody|Interferon inducible elF2 alpha kinase antibody|Interferon inducible RNA dependent protein kinase antibody|Interferon-induced, double-stranded RNA-activated protein kinase antibody|Interferon-inducible RNA-dependent protein kinase antibody|MGC126524 antibody|P1/eIF-2A protein kinase antibody|P1/eIF2A protein kinase antibody|p68 kinase antibody|PKR antibody|PPP1R83 antibody|PRKR antibody|Protein kinase interferon inducible double stranded RNA dependent antibody|Protein kinase RNA activated antibody|Protein kinase RNA-activated antibody|Protein phosphatase 1 regulatory subunit 83 antibody|Serine/threonine protein kinase TIK antibody|Tyrosine protein kinase EIF2AK2 antibody|Anti-PKR antibody [Y117] (ab32506) KO Validated

SCBT cat No: sc-136038|sc-136352|sc-393038|sc-6282|sc-708|sc-100378|sc-514626|sc-366778|sc-707|sc-366777|sc-1702|sc-709|sc-54313|sc-365696|

Name

Rabbit Polyclonal Phospho-EIF2AK2-T451 antibody

Catalogue No.

AP0340

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T451 of human EIF2AK2

Host

Rabbit

Applications

IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

68kDa

Formulation

Phospho-EIF2AK2-T451 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PKR antibody; PRKR antibody; EIF2AK1 antibody;

Protein names

PKR; PRKR; EIF2AK1;

Protein function

IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.

Protein tissue specificity

Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression.

Protein sequence and domain

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.; Contains 2 DRBM (double-stranded RNA-binding) domains.; Contains 1 protein kinase domain.

Protein post-translational modifications

Autophosphorylated on several Ser, Thr and Tyr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. Tyrosine autophosphorylation is essential for efficient dsRNA-binding, dimerization, and kinase activation.

Protein cellular localization

Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Nuclear localization is elevated in acute leukemia, myelodysplastic syndrome (MDS), melanoma, breast, colon, prostate and lung cancer patient samples or cell lines as well as neurocytes from advanced Creutzfeldt-Jakob disease patients.

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/PKR/">PKR</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-EIF2AK2-T451 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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