Rabbit Polyclonal Phospho-EIF4EBP1-S65 antibody (AP0032)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-EIF4EBP1-S65 antibody (AP0032)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

4E-BP1 antibody; 4EBP1 antibody; BP-1 antibody; MGC4316 antibody; PHAS-I antibody;
4E-BP1 antibody|4EBP1 antibody|4EBP1_HUMAN antibody|BP 1 antibody|eIF4E binding protein 1 antibody|eIF4E-binding protein 1 antibody|Eif4ebp1 antibody|Eukaryotic translation initiation factor 4E-binding protein 1 antibody|PHAS-I antibody|PHASI antibody|Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody|Anti-eIF4EBP1 antibody (ab2606)

SCBT cat No: sc-81149|sc-6024|sc-514073|sc-6025|sc-9977|sc-6936|sc-74397|sc-134232|sc-81175|sc-393788|sc-13454|sc-514810|sc-13453|sc-134233|

Name

Rabbit Polyclonal Phospho-EIF4EBP1-S65 antibody

Catalogue No.

AP0032

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S65 of human EIF4EBP1

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

Phospho-EIF4EBP1-S65 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

4E-BP1 antibody; 4EBP1 antibody; BP-1 antibody; MGC4316 antibody; PHAS-I antibody;

Protein names

4E-BP1; 4EBP1; BP-1; MGC4316; PHAS-I;

Protein function

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Protein sequence and domain

Belongs to the eIF4E-binding protein family. The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex.

Protein post-translational modifications

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.; Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Signaling-Intermediates/">Signaling Intermediates</a>><a href="http://antibodyplus.com/product-tag/4E-BP/">4E-BP</a><br><a href="http://antibodyplus.com/product-tag/Signaling-Intermediates/"&gt; (View all antibody categories related to Signaling Intermediates)</a>

Note

AntibodyPlus can customize Phospho-EIF4EBP1-S65 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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