Rabbit Polyclonal Phospho-EIF4EBP1-T45 antibody (AP0219)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-EIF4EBP1-T45 antibody (AP0219)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-EIF4EBP1-T45 antibody.

Alternative names:

Check alternative names for the antibody

Expand

BP-1 antibody; 4EBP1 antibody; 4E-BP1 antibody; PHAS-I antibody;
4E-BP1 antibody|4EBP1 antibody|4EBP1_HUMAN antibody|BP 1 antibody|eIF4E binding protein 1 antibody|eIF4E-binding protein 1 antibody|Eif4ebp1 antibody|Eukaryotic translation initiation factor 4E-binding protein 1 antibody|PHAS-I antibody|PHASI antibody|Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody|Anti-eIF4EBP1 antibody (ab2606)

SCBT cat No: sc-81149|sc-6024|sc-514073|sc-6025|sc-9977|sc-6936|sc-74397|sc-134232|sc-81175|sc-393788|sc-13454|sc-514810|sc-13453|sc-134233|

Name

Rabbit Polyclonal Phospho-EIF4EBP1-T45 antibody

Catalogue No.

AP0219

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding T45 of human EIF4EBP1

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

18kDa

Formulation

Phospho-EIF4EBP1-T45 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

BP-1 antibody; 4EBP1 antibody; 4E-BP1 antibody; PHAS-I antibody;

Protein names

BP-1; 4EBP1; 4E-BP1; PHAS-I;

Protein function

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Protein sequence and domain

Belongs to the eIF4E-binding protein family. The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex.

Protein post-translational modifications

Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.; Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Signaling-Intermediates/">Signaling Intermediates</a>><a href="http://antibodyplus.com/product-tag/4E-BP/">4E-BP</a><br><a href="http://antibodyplus.com/product-tag/Signaling-Intermediates/"&gt; (View all antibody categories related to Signaling Intermediates)</a>

Note

AntibodyPlus can customize Phospho-EIF4EBP1-T45 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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