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Rabbit Polyclonal Phospho-EphA2/3/4 (Y588/596) antibody (STJ90710)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for EphA2/3/4 (phospho Tyr588/596) Polyclonal Antibody.
Check alternative names for the antibodyExpand
EPHA2 antibody, ECK antibody,|ECK antibody|EPH Receptor A2 antibody|EPH Receptor A3 antibody|EPH Receptor A4 antibody|EPHA2 antibody|EPHA3 antibody|EPHA4 antibody|ETK1 antibody|HEK antibody|HEK4 antibody|HEK8 antibody|SEK antibody|TYRO4 antibody|Phospho anti-Eph receptor A2+A3+A4 (Y588 + Y596) antibody (ab62256)
SCBT cat No: sc-135897|sc-922|sc-25457|sc-921|
EphA2/3/4 (phospho Tyr588/596) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-EphA2/3/4 (Y588/596) Polyclonal Antibody detects endogenous levels of EphA2/3/4 protein only when phosphorylated at Y588/596.
Synthesized phospho-peptide derived from EphA2/3/4 (phospho Tyr588/596) at AA range 530-610
WB, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:20000;
|Molecular weight|| |
EphA2/3/4 (phospho Tyr588/596) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
EphA2/3/4 (phospho Tyr588/596) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Ephrin type-A receptor 2 antibody, Epithelial cell kinase antibody, Tyrosine-protein kinase receptor ECK antibody
|Protein names|| |
Ephrin type-A receptor 2 , Epithelial cell kinase , Tyrosine-protein kinase receptor ECK
|Protein function|| |
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
|Protein tissue specificity|| |
Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g. skin, intestine, lung, and ovary.
|Involvement in disease|| |
Cataract 6, multiple types (CTRCT6) [MIM:116600]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. CTRCT6 includes posterior polar and age-related cortical cataracts, among others. Posterior polar cataract is a subcapsular opacity, usually disk-shaped, located at the back of the lens. Age-related cortical cataract is a developmental punctate opacity restricted to the cortex. The cataract is white or cerulean, increases in number with age, but rarely affects vision. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: Overexpressed in several cancer types and promotes malignancy. .
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. / Contains 1 Eph LBD (Eph ligand-binding) domain. / Contains 2 fibronectin type-III domains. / Contains 1 protein kinase domain. / Contains 1 SAM (sterile alpha motif) domain.
|Protein post-translational modifications|| |
Autophosphorylates. Phosphorylated on tyrosine upon binding and activation by EFNA1. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit (PIK3R1, PIK3R2 or PIK3R3). These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and cell migration. Dephosphorylation of Tyr-930 by PTPRF prevents the interaction of EPHA2 with NCK1. Phosphorylated at Ser-897 by PKB; serum-induced phosphorylation which targets EPHA2 to the cell leading edge and stimulates cell migration. Phosphorylation by PKB is inhibited by EFNA1-activated EPHA2 which regulates PKB activity via a reciprocal regulatory loop. Dephosphorylated by ACP1. / Ubiquitinated by CHIP/STUB1. Ubiquitination is regulated by the HSP90 chaperone and regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation (By similarity).
|Protein cellular localization|| |
Cell membrane / Single-pass type I membrane protein / Cell projection > ruffle membrane / Single-pass type I membrane protein / Cell projection > lamellipodium membrane / Single-pass type I membrane protein / Cell junction > focal adhesion
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St John’s Laboratory Ltd.
|Product type|| |
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