Rabbit Polyclonal Phospho-Eps15 (Y849) antibody (STJ90629)


Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-Eps15 (Y849) antibody (STJ90629)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Eps15 (phospho Tyr849) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


EPS15 antibody, AF1P antibody,|AF 1P antibody|AF 1p protein antibody|AF1P antibody|ALL1 fused gene from chromosome 1 antibody|Epidermal growth factor receptor pathway substrate 15 antibody|Epidermal growth factor receptor substrate 15 antibody|EPS 15 antibody|MLLT 5 antibody|MLLT5 antibody|Protein Eps 15 antibody|Protein Eps15 antibody|Anti-EPS15 antibody [EP9280] (ab174291)
SCBT cat No: sc-135898|sc-534|sc-390259|sc-374578|sc-1840|sc-11716|



Eps15 (phospho Tyr849) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.


Synthesized phospho-peptide derived from Eps15 (phospho Tyr849) at AA range 790-870





Recommended dilution

WB 1:500-1:2000; ELISA 1:10000;







Molecular weight

100 kDa


Eps15 (phospho Tyr849) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


Eps15 (phospho Tyr849) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Epidermal growth factor receptor substrate 15 antibody, Protein Eps15 antibody, Protein AF-1p antibody

Database links

Human UniProt/Swiss-Prot:P42566;Mouse UniPort/Swiss-Prot: P42567;Rat UniProt/Swiss-Port: Q5JC29;Human Entrez Gene: 2060;Mouse Entrez Gene: 13858;Rat Entrez Gene: Rn.22316

Protein names

Epidermal growth factor receptor substrate 15 , Protein Eps15 , Protein AF-1p

Protein function

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

Protein tissue specificity

Ubiquitously expressed.

Involvement in disease

Note: A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with KMT2A/MLL1. The result is a rogue activator protein.

Protein sequence and domain

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins. / The UIM (ubiquitin-interacting motif) repeats specifically bind ‘Lys-33’-linked ubiquitin. / Contains 2 EF-hand domains. / Contains 3 EH domains. / Contains 2 UIM (ubiquitin-interacting motif) domains.

Protein post-translational modifications

Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR. / Ubiquitinated.

Protein cellular localization

Cytoplasm / Cell membrane; Peripheral membrane protein; Cytoplasmic side / Membrane > clathrin-coated pit / Early endosome membrane / Peripheral membrane protein / Cytoplasmic side


AntibodyPlus can customize Eps15 (phospho Tyr849) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below:

Welcome to AntibodyPlus!