Rabbit Polyclonal Phospho-ERK3-S189 antibody (AP0051)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-ERK3-S189 antibody (AP0051)

Supplier: ABclonal Inc.

Recommended applications: WB

Recommended dilution:

WB 1:500 – 1:2000

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-ERK3-S189 antibody.

Alternative names:

Check alternative names for the antibody

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DKFZp686F03189 antibody; ERK3 antibody; HsT17250 antibody; PRKM6 antibody; p97MAPK antibody; MAPK6 antibody;
ERK-3 antibody|ERK3 antibody|Extracellular signal regulated kinase 3 antibody|Extracellular signal regulated kinase p97 antibody|Extracellular signal-regulated kinase 3 antibody|HsT17250 antibody|MAP kinase 6 antibody|MAP kinase isoform p97 antibody|MAPK 6 antibody|MAPK6 antibody|Mitogen activated protein kinase 6 antibody|Mitogen-activated protein kinase 6 antibody|MK06_HUMAN antibody|p97 MAPK antibody|p97-MAPK antibody|PRKM6 antibody|Protein kinase mitogen activated 5 antibody|Protein kinase mitogen activated 6 antibody|Anti-MAPK6 antibody [EP1720Y] (ab53277)

SCBT cat No: sc-365234|sc-155|sc-374239|sc-393371|sc-156|

Name

Rabbit Polyclonal Phospho-ERK3-S189 antibody

Catalogue No.

AP0051

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S189 of human ERK3

Host

Rabbit

Applications

WB

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Formulation

Phospho-ERK3-S189 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

DKFZp686F03189 antibody; ERK3 antibody; HsT17250 antibody; PRKM6 antibody; p97MAPK antibody; MAPK6 antibody;

Protein names

DKFZp686F03189; ERK3; HsT17250; PRKM6; p97MAPK; MAPK6;

Protein function

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

Protein tissue specificity

Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.

Protein sequence and domain

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.; Contains 1 protein kinase domain. In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.

Protein post-translational modifications

Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.; Ubiquitination at Met-1 leads to degradation by the proteasome pathway.

Protein cellular localization

Cytoplasm . Nucleus . Note=Translocates to the cytoplasm following interaction with MAPKAPK5.

Background

Erk3, also known as MAPK6 or p97 MAPK, is almost 50% identical to Erk1/2 at the kinase domain located in its amino-terminal region (1). However, Erk3 is distinguished from other MAP kinases in that it lacks the conserved TXY motif in its activation loop, possessing instead an SEG motif (1,2). Phosphorylation at Ser189 in the SEG motif has been reported (2,3). With limited information about its upstream kinases and downstream substrates, the significance of this phosphorylation remains to be elucidated (3,4). Erk3 is an inherently unstable protein, rapidly degraded through amino-terminal ubiquitination and proteasome degradation (3,5). A site-specific cleavage, depending on a short stretch of acidic residues of Erk3, might regulate its translocation from the Golgi/ERGIC to the nucleus during the cell cycle (6). Accumulating evidence suggests that Erk3 is involved in cell differentiation (1,3,6).

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Kinases-and-Phosphatases/">Kinases and Phosphatases</a>><a href="http://antibodyplus.com/product-tag/ERK/">ERK</a><br><a href="http://antibodyplus.com/product-tag/Kinases-and-Phosphatases/"&gt; (View all antibody categories related to Kinases and Phosphatases)</a>

Note

AntibodyPlus can customize Phospho-ERK3-S189 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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