Rabbit Polyclonal Phospho-Ezrin (Y353) antibody (STJ90267)

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Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Ezrin (Y353) antibody (STJ90267)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Ezrin (phospho Tyr353) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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EZR antibody, VIL2 antibody,|Villin 2 ezrin antibody|CVIL antibody|CVL antibody|Cytovillin 2 antibody|Cytovillin antibody|DKFZp762H157 antibody|Epididymis secretory protein Li 105 antibody|EZR antibody|EZRI_HUMAN antibody|Ezrin antibody|FLJ26216 antibody|HEL S 105 antibody|MGC1584 antibody|p81 antibody|VIL 2 antibody|VIL2 antibody|Villin 2 (ezrin) antibody|Villin 2 antibody|Villin-2 antibody|Villin2 antibody|Anti-Ezrin antibody [3C12] (ab4069)
SCBT cat No: sc-58758|sc-32759|sc-71080|sc-71081|sc-71082|sc-271456|sc-6409|sc-6407|sc-271541|sc-398807|sc-377205|sc-20773|sc-32918|sc-398542|sc-56388|sc-71086|sc-71088|sc-71087|

 

Name

Ezrin (phospho Tyr353) Polyclonal Antibody

Catalogue No.

STJ90267

Reactivity

Human, Mouse, Rat

Specificity

Phospho-Ezrin (Y353) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at Y353.

Immunogen

Synthesized phospho-peptide derived from Ezrin (phospho Tyr353) at AA range 290-370

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

69 kDa

Formulation

Ezrin (phospho Tyr353) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Ezrin (phospho Tyr353) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Ezrin antibody, Cytovillin antibody, Villin-2 antibody, p81 antibody

Database links

Human UniProt/Swiss-Prot:P15311;Mouse UniPort/Swiss-Prot: P26040;Rat UniProt/Swiss-Port: P31977;Human Entrez Gene: 7430;Mouse Entrez Gene: 22350;Rat Entrez Gene: Rn.773

Protein names

Ezrin , Cytovillin , Villin-2 , p81

Protein function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. / A head-to-tail association, of the N-terminal and C-terminal halves results in a closed conformation (inactive form) which is incapable of actin or membrane-binding.

Protein tissue specificity

Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied.

Protein sequence and domain

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. / Contains 1 FERM domain.

Protein post-translational modifications

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). / S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Protein cellular localization

Apical cell membrane / Peripheral membrane protein / Cytoplasmic side / Cell projection / Cell projection > microvillus membrane / Peripheral membrane protein / Cytoplasmic side / Cell projection > ruffle membrane / Peripheral membrane protein / Cytoplasmic side / Cytoplasm > cell cortex / Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Ezrin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize Ezrin (phospho Tyr353) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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