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Rabbit Polyclonal Phospho-FANCD2 (S222) antibody (STJ90790)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for FANCD2 (phospho Ser222) Polyclonal Antibody.
Check alternative names for the antibodyExpand
FANCD2 antibody, FACD antibody,|DKFZp762A223 antibody|FA 4 antibody|FA D2 antibody|FA4 antibody|FAC D2 antibody|FACD 2 antibody|FACD antibody|FACD2 antibody|FACD2_HUMAN antibody|FAD antibody|FAD2 antibody|FANC D2 antibody|FANCD 2 antibody|FANCD antibody|FANCD2 antibody|FANCONI ANEMIA COMPLEMENTATION GROUP D antibody|Fanconi anemia complementation group D2 antibody|Fanconi anemia group D2 protein antibody|FANCONI PANCYTOPENIA TYPE 4 antibody|FLJ23826 antibody|OTTHUMP00000158853 antibody|OTTHUMP00000207925 antibody|Protein FACD2 antibody|Type 4 Fanconi pancytopenia antibody|Anti-FANCD2 antibody [EPR2302] (ab108928)
SCBT cat No: sc-23584|sc-20022|sc-28194|
FANCD2 (phospho Ser222) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-FANCD2 (S222) Polyclonal Antibody detects endogenous levels of FANCD2 protein only when phosphorylated at S222.
Synthesized phospho-peptide derived from FANCD2 (phospho Ser222) at AA range 160-240
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
FANCD2 (phospho Ser222) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
FANCD2 (phospho Ser222) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Fanconi anemia group D2 protein antibody, Protein FACD2 antibody
|Protein names|| |
Fanconi anemia group D2 protein , Protein FACD2
|Protein function|| |
Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
|Protein tissue specificity|| |
Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
|Involvement in disease|| |
Fanconi anemia complementation group D2 (FANCD2) [MIM:227646]: A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The C-terminal 24 residues of isoform 2 are required for its function.
|Protein post-translational modifications|| |
Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1. / Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
|Protein cellular localization|| |
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St John’s Laboratory Ltd.
|Product type|| |
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