Rabbit Polyclonal Phospho-Filamin 1 (S2152) antibody (STJ90760)

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Reactivity: Human, Mouse, Rat, Monkey
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Filamin 1 (S2152) antibody (STJ90760)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Filamin 1 (phospho Ser2152) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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FLNA antibody, FLN antibody, FLN1 antibody,|ABP 280 antibody|ABP-280 antibody|Actin-binding protein 280 antibody|Alpha filamin antibody|Alpha-filamin antibody|APBX antibody|CSBS antibody|CVD1 antibody|Endothelial actin binding protein antibody|Endothelial actin-binding protein antibody|Filamin 1 antibody|Filamin A alpha antibody|Filamin A antibody|Filamin-1 antibody|Filamin-A antibody|FLN antibody|FLN-A antibody|FLN1 antibody|FLNA antibody|FLNA_HUMAN antibody|FMD antibody|MNS antibody|NHBP antibody|Non muscle filamin antibody|Non-muscle filamin antibody|OPD antibody|OPD1 antibody|OPD2 antibody|XLVD antibody|XMVD antibody|Anti-Filamin A antibody [EP2405Y] (ab76289)
SCBT cat No: sc-58762|sc-71118|sc-135906|sc-17749|sc-58763|sc-271440|sc-28284|sc-7565|sc-58764|

 

Name

Filamin 1 (phospho Ser2152) Polyclonal Antibody

Catalogue No.

STJ90760

Reactivity

Human, Mouse, Rat, Monkey

Specificity

Phospho-Filamin 1 (S2152) Polyclonal Antibody detects endogenous levels of Filamin 1 protein only when phosphorylated at S2152.

Immunogen

Synthesized phospho-peptide derived from Filamin 1 (phospho Ser2152) at AA range 2090-2170

Host

Rabbit

Applications

WB, IHC, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

280 kDa

Formulation

Filamin 1 (phospho Ser2152) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Filamin 1 (phospho Ser2152) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Filamin-A antibody, FLN-A antibody, Actin-binding protein 280 antibody, ABP-280 antibody, Alpha-filamin antibody, Endothelial actin-binding protein antibody, Filamin-1 antibody, Non-muscle filamin antibody

Database links

Human UniProt/Swiss-Prot:P21333;Mouse UniPort/Swiss-Prot: Q8BTM8;Rat UniProt/Swiss-Port: C0JPT7;Human Entrez Gene: 2316;Mouse Entrez Gene: 192176;Rat Entrez Gene: Rn.4213

Protein names

Filamin-A , FLN-A , Actin-binding protein 280 , ABP-280 , Alpha-filamin , Endothelial actin-binding protein , Filamin-1 , Non-muscle filamin

Protein function

Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking (By similarity). Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs. Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintainance (By similarity).

Protein tissue specificity

Ubiquitous.

Involvement in disease

Periventricular nodular heterotopia 1 (PVNH1) [MIM:300049]: A developmental disorder characterized by the presence of periventricular nodules of cerebral gray matter, resulting from a failure of neurons to migrate normally from the lateral ventricular proliferative zone, where they are formed, to the cerebral cortex. PVNH1 is an X-linked dominant form. Heterozygous females have normal intelligence but suffer from seizures and various manifestations outside the central nervous system, especially related to the vascular system. Hemizygous affected males die in the prenatal or perinatal period. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Otopalatodigital syndrome 1 (OPD1) [MIM:311300]: X-linked dominant multiple congenital anomalies disease mainly characterized by a generalized skeletal dysplasia, mild mental retardation, hearing loss, cleft palate, and typical facial anomalies. OPD1 belongs to a group of X-linked skeletal dysplasias known as oto-palato-digital syndrome spectrum disorders that also include OPD2, Melnick-Needles syndrome (MNS), and frontometaphyseal dysplasia (FMD). Remodeling of the cytoskeleton is central to the modulation of cell shape and migration. FLNA is a widely expressed protein that regulates re-organization of the actin cytoskeleton by interacting with integrins, transmembrane receptor complexes and second messengers. Males with OPD1 have cleft palate, malformations of the ossicles causing deafness and milder bone and limb defects than those associated with OPD2. Obligate female carriers of mutations causing both OPD1 and OPD2 have variable (often milder) expression of a similar phenotypic spectrum. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Otopalatodigital syndrome 2 (OPD2) [MIM:304120]: Congenital bone disorder that is characterized by abnormally modeled, bowed bones, small or absent first digits and, more variably, cleft palate, posterior fossa brain anomalies, omphalocele and cardiac defects. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Frontometaphyseal dysplasia (FMD) [MIM:305620]: Congenital bone disease characterized by supraorbital hyperostosis, deafness and digital anomalies. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Melnick-Needles syndrome (MNS) [MIM:309350]: Severe congenital bone disorder characterized by typical facies (exophthalmos, full cheeks, micrognathia and malalignment of teeth), flaring of the metaphyses of long bones, s-like curvature of bones of legs, irregular constrictions in the ribs, and sclerosis of base of skull. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Intestinal pseudoobstruction, neuronal, chronic idiopathic, X-linked (IPOX) [MIM:300048]: A disease characterized by a severe abnormality of gastrointestinal motility due to primary qualitative defects of enteric ganglia and nerve fibers. Affected individuals manifest recurrent signs of intestinal obstruction in the absence of any mechanical lesion. . Note: The disease is caused by mutations affecting the gene represented in this entry.; FG syndrome 2 (FGS2) [MIM:300321]: FG syndrome (FGS) is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Terminal osseous dysplasia (TOD) [MIM:300244]: A rare X-linked dominant male-lethal disease characterized by skeletal dysplasia of the limbs, pigmentary defects of the skin and recurrent digital fibroma during infancy. A significant phenotypic variability is observed in affected females. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Cardiac valvular dysplasia X-linked (CVDX) [MIM:314400]: A rare X-linked heart disease characterized by mitral and/or aortic valve regurgitation. The histologic features include fragmentation of collagenous bundles within the valve fibrosa and accumulation of proteoglycans, which produces excessive valve tissue leading to billowing of the valve leaflets. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Note: Defects in FLNA may be a cause of macrothrombocytopenia, a disorder characterized by subnormal levels of blood platelets. Blood platelets are abnormally enlarged (PubMed:21960593). .; Congenital short bowel syndrome, X-linked (CSBSX) [MIM:300048]: A disease characterized by a shortened small intestine, and malabsorption. The mean length of the small intestine in affected individuals is approximately 50 cm, compared with a normal length at birth of 190-280 cm. It is associated with significant mortality and morbidity. Infants usually present with failure to thrive, recurrent vomiting, and diarrhea. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Comprised of a NH2-terminal actin-binding domain, 24 immunoglobulin-like internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. Filamin repeat 20 interacts with filamin repeat 21 masking the ligand binding site on filamin repeat 21, resulting in an autoinhibited conformation (PubMed:17690686). The autoinhibition can be relieved by ligands like ITGB7 or FBLIM1 (PubMed:21524097). Filamin repeats 19 and 21 can simultaneously engage ligands (PubMed:21524097). / Belongs to the filamin family. / Contains 1 actin-binding domain. / Contains 2 CH (calponin-homology) domains. / Contains 24 filamin repeats.

Protein post-translational modifications

Phosphorylation at Ser-2152 is negatively regulated by the autoinhibited conformation of filamin repeats 19-21. Ligand binding induces a conformational switch triggering phosphorylation at Ser-2152 by PKA. / Phosphorylation extent changes in response to cell activation. / Polyubiquitination in the CH1 domain by a SCF-like complex containing ASB2 leads to proteasomal degradation. Prior dissociation from actin may be required to expose the target lysines . Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome .

Protein cellular localization

Cytoplasm > cell cortex / Cytoplasm > cytoskeleton

Research area

All research areas>Structural Proteins>Filamin
(View all antibody categories related to Structural Proteins)

Note

AntibodyPlus can customize Filamin 1 (phospho Ser2152) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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