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Rabbit Polyclonal Phospho-Flt3 (Y599) antibody (STJ90464)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for Flt3 (phospho Tyr599) Polyclonal Antibody.
Check alternative names for the antibodyExpand
FLT3 antibody, CD135 antibody, FLK2 antibody, STK1 antibody,|CD 135 antibody|Fms-like tyrosine kinase 3 antibody|Growth factor receptor tyrosine kinase type III antibody|Ly-72 antibody|OTTHUMP0000004234 antibody|Receptor type tyrosine protein kinase FLT3 antibody|Stem cell tyrosine kinase 1 antibody|Stk 1 antibody|STK-1 antibody|Stk1 antibody|Tyrosine protein kinase receptor FLT3 antibody|Tyrosine-protein kinase receptor FLT3 antibody|Anti-Flt3 / CD135 antibody – Aminoterminal end (ab37847)
SCBT cat No: sc-101343|sc-21788|sc-479|sc-20733|sc-340|sc-480|
Flt3 (phospho Tyr599) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Monkey
Phospho-Flt3 (Y599) Polyclonal Antibody detects endogenous levels of Flt3 protein only when phosphorylated at Y599.
Synthesized phospho-peptide derived from Flt3 (phospho Tyr599) at AA range 540-620
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:5000;
|Molecular weight|| |
Flt3 (phospho Tyr599) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Flt3 (phospho Tyr599) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Receptor-type tyrosine-protein kinase FLT3 antibody, FL cytokine receptor antibody, Fetal liver kinase-2 antibody, FLK-2 antibody, Fms-like tyrosine kinase 3 antibody, FLT-3 antibody, Stem cell tyrosine kinase 1 antibody, STK-1 antibody, CD antigen CD135 antibody
|Protein names|| |
Receptor-type tyrosine-protein kinase FLT3 , FL cytokine receptor , Fetal liver kinase-2 , FLK-2 , Fms-like tyrosine kinase 3 , FLT-3 , Stem cell tyrosine kinase 1 , STK-1 , CD antigen CD135
|Protein function|| |
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Present in an inactive conformation in the absence of bound ligand. FLT3LG binding leads to dimerization and activation by autophosphorylation.
|Protein tissue specificity|| |
Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.
|Involvement in disease|| |
Leukemia, acute myelogenous (AML) [MIM:601626]: A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. . Note: The gene represented in this entry may be involved in disease pathogenesis. Somatic mutations that lead to constitutive activation of FLT3 are frequent in AML patients. These mutations fall into two classes, the most common being in-frame internal tandem duplications of variable length in the juxtamembrane region that disrupt the normal regulation of the kinase activity. Likewise, point mutations in the activation loop of the kinase domain can result in a constitutively activated kinase.
|Protein sequence and domain|| |
The juxtamembrane autoregulatory region is important for normal regulation of the kinase activity and for maintaining the kinase in an inactive state in the absence of bound ligand. Upon tyrosine phosphorylation, it mediates interaction with the SH2 domains of numerous signaling partners. In-frame internal tandem duplications (ITDs) result in constitutive activation of the kinase. The activity of the mutant kinase can be stimulated further by FLT3LG binding. / Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. / Contains 1 Ig-like C2-type (immunoglobulin-like) domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
N-glycosylated, contains complex N-glycans with sialic acid. / Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane. / Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation.
|Protein cellular localization|| |
Membrane; Single-pass type I membrane protein / Endoplasmic reticulum lumen
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St John’s Laboratory Ltd.
|Product type|| |
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