Rabbit Polyclonal Phospho-FoxO3A (S253) antibody (STJ90275)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, IHC
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-FoxO3A (S253) antibody (STJ90275)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for FoxO3A (phospho Ser253) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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FOXO3 antibody, FKHRL1 antibody, FOXO3A antibody,|AFX1 antibody|FKH1 antibody|FKHRL1 antibody|FKHRL1P2 antibody|Forkhead box O1 antibody|Forkhead box O3 antibody|Forkhead box O4 antibody|FOXO1 antibody|FOXO2 antibody|FOXO3 antibody|FOXO4 antibody|MLLT7 antibody|Phospho anti-FOXO1 + FOXO3 + FOXO4 (T24 + T32) antibody (ab58517)
SCBT cat No: sc-34894|sc-34895|sc-48348|sc-11351|sc-34897|sc-9813|sc-9812|sc-48503|sc-374642|sc-48506|

Name

FoxO3A (phospho Ser253) Polyclonal Antibody

Catalogue No.

STJ90275

Reactivity

Human, Mouse, Rat

Specificity

Phospho-FoxO3A (S253) Polyclonal Antibody detects endogenous levels of FoxO3A protein only when phosphorylated at S253.

Immunogen

Synthesized phospho-peptide derived from FoxO3A (phospho Ser253) at AA range 190-270

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

71 kDa

Formulation

FoxO3A (phospho Ser253) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

FoxO3A (phospho Ser253) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Forkhead box protein O3 antibody, AF6q21 protein antibody, Forkhead in rhabdomyosarcoma-like 1 antibody

Database links

Human UniProt/Swiss-Prot:O43524;Mouse UniPort/Swiss-Prot: Q9WVH4;Rat UniProt/Swiss-Port: D3ZBQ1;Human Entrez Gene: 2309;Mouse Entrez Gene: 56484;Rat Entrez Gene: Rn.165338

Protein names

Forkhead box protein O3 , AF6q21 protein , Forkhead in rhabdomyosarcoma-like 1

Protein function

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5′-[AG]TAAA[TC]A-3′. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation.

Protein tissue specificity

Ubiquitous.

Involvement in disease

Note: A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with KMT2A/MLL1.

Protein sequence and domain

Contains 1 fork-head DNA-binding domain.

Protein post-translational modifications

In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn’t appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4/MST1 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. Phosphorylation by AMPK leads to the activation of transcriptional activity without affecting subcellular localization. Phosphorylation by MAPKAPK5 promotes nuclear localization and DNA-binding, leading to induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3’UTR of MYC transcript and prevent its translation . Phosphorylated by CHUK/IKKA and IKBKB/IKKB. TNF-induced inactivation of FOXO3 requires its phosphorylation at Ser-644 by IKBKB/IKKB which promotes FOXO3 retention in the cytoplasm, polyubiquitination and ubiquitin-mediated proteasomal degradation . / Deacetylation by SIRT1 or SIRT2 stimulates interaction of FOXO3 with SKP2 and facilitates SCF(SKP2)-mediated FOXO3 ubiquitination and proteasomal degradation . Deacetylation by SIRT2 stimulates FOXO3-mediated transcriptional activity in response to oxidative stress (By similarity). / Heavily methylated by SET9 which decreases stability, while moderately increasing transcriptional activity. The main methylation site is Lys-271. Methylation doesn’t affect subcellular location. / Polyubiquitinated. Ubiquitinated by a SCF complex containing SKP2, leading to proteasomal degradation.

Protein cellular localization

Cytoplasm > cytosol / Nucleus

Research area

All research areas>Transcription Regulators>FKHR
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize FoxO3A (phospho Ser253) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

Reviews


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Antibody customer reviews & validation data - 201

Validation data ID: 201

Submission date: 2017-06-29

View Details

Result figure:
p-Foxo3a.pdf

Figure description:

Human multiple myeloma cell line KMS11 was cultured in RPMI-8226 media and then lysates are made in RIPA buffer supplemented with phosphatase and proteinase inhibitors. 2ug of protein was loaded in two wells and electrophoresed for 30min at 200V BIORAD electrophoresis unit. Gel was transferred to PVDF membrane and blot evaluated for the expression of p-FOXO3a.

Product name

Rabbit Polyclonal Phospho-FoxO3A (S253) antibody

Product catalog No.

STJ90275

Product type

Primary antibody

Supplier

St John's Laboratory

Application type

Western Blot(WB)

Antibody specificity rating

Good

Antibody overall rating

Good

Your comments

This antibody worked well for me. I had tried antibodies from other companies also but only this worked.

Detailed protocol

View Details

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Immunohistochemical analysis of paraffin embedded Human colon tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat testis tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat lung tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Rat spleen tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse testis tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse liver tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
 

Immunohistochemical analysis of paraffin embedded Mouse colon tissue

1: FoxO3A (phospho Ser253) Polyclonal Antibody was diluted at 1:200 (4 degree Celsius,overnight).
2: Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree Celsius,20min).
3: Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.


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