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Rabbit Polyclonal Phospho-Fyn (Y530) antibody (STJ90792)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for Fyn (phospho Tyr530) Polyclonal Antibody.
Check alternative names for the antibodyExpand
FYN antibody,|C syn protooncogene antibody|FYN_HUMAN antibody|Src-like kinase antibody|SYN antibody|Tyrosine kinase p59fyn T antibody|Tyrosine kinase p59fyn(T) antibody|Tyrosine-protein kinase Fyn antibody|Anti-Fyn antibody [FYN-01] (ab1881)
SCBT cat No: sc-8056|sc-5266|sc-19|sc-18|sc-167555|sc-383868|sc-167557|sc-393260|sc-393962|sc-393317|sc-166647|sc-16473|sc-398840|sc-398862|
Fyn (phospho Tyr530) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Fyn (Y530) Polyclonal Antibody detects endogenous levels of Fyn protein only when phosphorylated at Y530.
Synthesized phospho-peptide derived from Fyn (phospho Tyr530) at AA range 460-540
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
Fyn (phospho Tyr530) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Fyn (phospho Tyr530) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Tyrosine-protein kinase Fyn antibody, Proto-oncogene Syn antibody, Proto-oncogene c-Fyn antibody, Src-like kinase antibody, SLK antibody, p59-Fyn antibody
|Protein names|| |
Tyrosine-protein kinase Fyn , Proto-oncogene Syn , Proto-oncogene c-Fyn , Src-like kinase , SLK , p59-Fyn
|Protein function|| |
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Mn2+ / Inhibited by phosphorylation of Tyr-531 by leukocyte common antigen and activated by dephosphorylation of this site.
|Protein tissue specificity|| |
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. / Contains 1 protein kinase domain. / Contains 1 SH2 domain. / Contains 1 SH3 domain.
|Protein post-translational modifications|| |
Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (By similarity). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. / Palmitoylation at Cys-3 and Cys-6 regulates subcellular location.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Cell membrane
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St John’s Laboratory Ltd.
|Product type|| |
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