Rabbit Polyclonal Phospho-G3BP1-S232 antibody (AP0352)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-G3BP1-S232 antibody (AP0352)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100
IF 1:100 – 1:200

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

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G3BP antibody; HDH-VIII antibody;
ATP dependent DNA helicase VIII antibody|ATP-dependent DNA helicase VIII antibody|G3BP antibody|G3BP-1 antibody|G3bp1 antibody|G3BP1_HUMAN antibody|GAP binding protein antibody|GAP SH3 domain binding protein 1 antibody|GAP SH3 domain-binding protein 1 antibody|GTPase activating protein (SH3 domain) binding protein 1 antibody|hDH VIII antibody|Human DNA helicase VIII antibody|MGC111040 antibody|Ras GTPase activating protein binding protein 1 antibody|Ras GTPase activating protein SH3 domain binding protein antibody|Ras GTPase-activating protein-binding protein 1 antibody|RasGAP associated endoribonuclease G3BP antibody|Anti-G3BP antibody (ab56574)

SCBT cat No: sc-70283|sc-365338|sc-98561|sc-70286|sc-81940|sc-161612|

Name

Rabbit Polyclonal Phospho-G3BP1-S232 antibody

Catalogue No.

AP0352

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S232 of human G3BP1

Host

Rabbit

Applications

WB, IHC, IF

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

60kDa

Formulation

Phospho-G3BP1-S232 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

G3BP antibody; HDH-VIII antibody;

Protein names

G3BP; HDH-VIII;

Protein function

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Contains 1 NTF2 domain.; Contains 1 RRM (RNA recognition motif) domain. The NTF2 domain mediates multimerization.

Protein post-translational modifications

Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.; Arg-435 is dimethylated, probably to asymmetric dimethylarginine.

Protein cellular localization

Cytoplasm. Cytoplasm, cytosol. Cytoplasmic granule. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.

Background

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/GDPGTP-Binding-Proteins/">GDP/GTP Binding Proteins</a>><a href="http://antibodyplus.com/product-tag/G3BP/">G3BP</a><br><a href="http://antibodyplus.com/product-tag/GDPGTP-Binding-Proteins/"&gt; (View all antibody categories related to GDP/GTP Binding Proteins)</a>

Note

AntibodyPlus can customize Phospho-G3BP1-S232 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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