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Rabbit Polyclonal Phospho-G3BP1 (S232) antibody (STJ90277)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for G3BP1 (phospho Ser232) Polyclonal Antibody.
Check alternative names for the antibodyExpand
G3BP1 antibody, G3BP antibody,|ATP dependent DNA helicase VIII antibody|ATP-dependent DNA helicase VIII antibody|G3BP antibody|G3BP-1 antibody|G3bp1 antibody|G3BP1_HUMAN antibody|GAP binding protein antibody|GAP SH3 domain binding protein 1 antibody|GAP SH3 domain-binding protein 1 antibody|GTPase activating protein (SH3 domain) binding protein 1 antibody|hDH VIII antibody|Human DNA helicase VIII antibody|MGC111040 antibody|Ras GTPase activating protein binding protein 1 antibody|Ras GTPase activating protein SH3 domain binding protein antibody|Ras GTPase-activating protein-binding protein 1 antibody|RasGAP associated endoribonuclease G3BP antibody|Anti-G3BP antibody (ab56574)
SCBT cat No: sc-70283|sc-365338|sc-98561|sc-70286|sc-81940|sc-161612|
G3BP1 (phospho Ser232) Polyclonal Antibody
|Catalogue No.|| |
Phospho-G3BP1 (S232) Polyclonal Antibody detects endogenous levels of G3BP1 protein only when phosphorylated at S232.
Synthesized phospho-peptide derived from G3BP1 (phospho Ser232) at AA range 170-250
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
G3BP1 (phospho Ser232) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
G3BP1 (phospho Ser232) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Ras GTPase-activating protein-binding protein 1 antibody, G3BP-1 antibody, ATP-dependent DNA helicase VIII antibody, hDH VIII antibody, GAP SH3 domain-binding protein 1 antibody
|Protein names|| |
Ras GTPase-activating protein-binding protein 1 , G3BP-1 , ATP-dependent DNA helicase VIII , hDH VIII , GAP SH3 domain-binding protein 1
|Protein function|| |
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3′-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3′ tail or hanging tails at both 5′- and 3′-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5′ to 3′ direction along the bound single-stranded DNA. / ATP + H2O = ADP + phosphate. / Mg2+
|Protein tissue specificity|| |
|Protein sequence and domain|| |
The NTF2 domain mediates multimerization. / Contains 1 NTF2 domain. / Contains 1 RRM (RNA recognition motif) domain.
|Protein post-translational modifications|| |
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. / Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
|Protein cellular localization|| |
Cytoplasm / Cytoplasm > cytosol / Cytoplasmic granule / Cell membrane / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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