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Rabbit Polyclonal Phospho-Hck (Y521) antibody (STJ90654)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for Hck (phospho Tyr521) Polyclonal Antibody.
Check alternative names for the antibodyExpand
HCK antibody,|Bmk antibody|Hck 1 antibody|Hck antibody|HCK_HUMAN antibody|Hemopoietic cell kinase antibody|JTK9 antibody|p59-HCK/p60-HCK antibody|p59Hck antibody|p59HCK/p60HCK antibody|p61Hck antibody|Tyrosine protein kinase HCK antibody|Tyrosine-protein kinase HCK antibody|Anti-Hck antibody – N-terminal (ab175886)
SCBT cat No: sc-1428|
Hck (phospho Tyr521) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-Hck (Y521) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y521.
Synthesized phospho-peptide derived from Hck (phospho Tyr521) at AA range 450-530
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:10000;
|Molecular weight|| |
Hck (phospho Tyr521) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Hck (phospho Tyr521) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Tyrosine-protein kinase HCK antibody, Hematopoietic cell kinase antibody, Hemopoietic cell kinase antibody, p59-HCK/p60-HCK antibody, p59Hck antibody, p61Hck antibody
|Protein names|| |
Tyrosine-protein kinase HCK , Hematopoietic cell kinase , Hemopoietic cell kinase , p59-HCK/p60-HCK , p59Hck , p61Hck
|Protein function|| |
Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS. / ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. / Subject to autoinhibition, mediated by intramolecular interactions involving the SH2 and SH3 domains. Kinase activity is also regulated by phosphorylation at regulatory tyrosine residues. Phosphorylation at Tyr-411 is required for optimal activity. Phosphorylation at Tyr-522 inhibits kinase activity. Inhibited by PP1 and A-770041.
|Protein tissue specificity|| |
Detected in monocytes and neutrophils (at protein level). Expressed predominantly in cells of the myeloid and B-lymphoid lineages. Highly expressed in granulocytes. Detected in tonsil.
|Involvement in disease|| |
Note: Aberrant activation of HCK by HIV-1 protein Nef enhances HIV-1 replication and contributes to HIV-1 pathogenicity.; Note: Aberrant activation of HCK, e.g. by the BCR-ABL fusion protein, promotes cancer cell proliferation.
|Protein sequence and domain|| |
The SH3 domain mediates binding to HIV-1 Nef. / Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. / Contains 1 protein kinase domain. / Contains 1 SH2 domain. / Contains 1 SH3 domain.
|Protein post-translational modifications|| |
Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases. / Ubiquitinated by CBL, leading to its degradation via the proteasome. / Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.
|Protein cellular localization|| |
Lysosome / Membrane; Lipid-anchor / Cell projection > podosome membrane; Lipid-anchor / Cytoplasm > cytosol / Cell membrane / Lipid-anchor / Membrane > caveola / Lipid-anchor / Cell junction > focal adhesion / Cytoplasm > cytoskeleton / Golgi apparatus / Cytoplasmic vesicle / Lysosome / Nucleus / Cytoplasmic vesicle > secretory vesicle / Cytoplasm > cytosol
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St John’s Laboratory Ltd.
|Product type|| |
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