Rabbit Polyclonal Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 antibody (AP0280)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

Clear
-$50 as credit for submitting review for primary antibody
Shipping notice:
Product will be delivered in 6 to 8 Business Days

Rabbit Polyclonal Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 antibody (AP0280)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:1000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 antibody.

Alternative names:

Check alternative names for the antibody

Expand

HD4 antibody; AHO3 antibody; BDMR antibody; HDACA antibody; HA6116 antibody; HDAC-4 antibody; HDAC-A/HD5 antibody;NY-CO-9/HD7 antibody; HD9 antibody; HD7b antibody; HDAC antibody; HDRP antibody; MITR antibody; HDAC7 antibody; HDAC7B antibody;HDAC9B antibody; HDAC9FL
AHO3 antibody|MEF2-interacting transcription repressor MITR antibody|MITR antibody|NY-CO-9 antibody|Anti-HDAC4 antibody (ab12172)

SCBT cat No: sc-46672|sc-365093|sc-48390|sc-365367|sc-11418|sc-56686|sc-5246|sc-5245|sc-11421|

Name

Rabbit Polyclonal Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 antibody

Catalogue No.

AP0280

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S246/259/220 of human HDAC4/5/9

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

140/142kDa

Formulation

Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HD4 antibody; AHO3 antibody; BDMR antibody; HDACA antibody; HA6116 antibody; HDAC-4 antibody; HDAC-A/HD5 antibody;NY-CO-9/HD7 antibody; HD9 antibody; HD7b antibody; HDAC antibody; HDRP antibody; MITR antibody; HDAC7 antibody; HDAC7B antibody;HDAC9B antibody; HDAC9FL

Protein names

HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A/HD5;NY-CO-9/HD7; HD9; HD7b; HDAC; HDRP; MITR; HDAC7; HDAC7B;HDAC9B; HDAC9FL

Protein function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Belongs to the histone deacetylase family. HD type 2 subfamily. The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.; The PxLPxI/L motif mediates interaction with ankyrin repeats of ANKRA2.

Protein post-translational modifications

Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues by CaMK2D is required for the interaction with 14-3-3. Phosphorylation at Ser-350, within the PxLPxI/L motif, impairs the binding of ANKRA2 but generates a high-affinity docking site for 14-3-3.; Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

Protein cellular localization

Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4 and SIK1. The nuclear localization probably depends on sumoylation.

Background

Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/HDAC/">HDAC</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"&gt; (View all antibody categories related to Transcription Regulators)</a>

Note

AntibodyPlus can customize Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

Reviews


There are no reviews yet.

Only logged in customers who have purchased this product may leave a review

There are no reviews for this product yet.
By submitting a review, get following benefits:

1. Receive $50 ABcoins as credit for each review.
2. First trial sample order will be fully refunded as credit.
3. Have a chance to win a $50 amazon gift card!

view database submit reviews

There is no extra validation for this product yet.
Check other extra validated antibodies below: