Rabbit Polyclonal Phospho-HDAC5-S498 antibody (AP0202)

$99.00$259.00

Reactivity: Human,Mouse,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-HDAC5-S498 antibody (AP0202)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-HDAC5-S498 antibody.

Alternative names:

Check alternative names for the antibody

Expand

HD5 antibody; NY-CO-9 antibody;
Antigen NY CO 9 antibody|Antigen NY-CO-9 antibody|HD5 antibody|HDAC 5 antibody|HDAC5 antibody|HDAC5_HUMAN antibody|Histone deacetylase 5 antibody|NY CO 9 antibody|Anti-HDAC5 antibody (ab1439)

SCBT cat No: sc-133106|sc-133225|sc-5250|sc-11419|sc-5252|sc-28386|

Name

Rabbit Polyclonal Phospho-HDAC5-S498 antibody

Catalogue No.

AP0202

Reactivity

Human, Mouse, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S498 of human HDAC5

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

124kDa

Formulation

Phospho-HDAC5-S498 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HD5 antibody; NY-CO-9 antibody;

Protein names

HD5; NY-CO-9;

Protein function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Protein tissue specificity

Ubiquitous.

Protein sequence and domain

Belongs to the histone deacetylase family. HD type 2 subfamily. The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.

Protein post-translational modifications

Phosphorylated by AMPK, CaMK1, SIK1 and PRKD1 at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm and inhibition. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Phosphorylated by GRK5, leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription (By similarity).; Ubiquitinated. Polyubiquitination however does not lead to its degradation.

Protein cellular localization

Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by AMPK, CaMK1 and SIK1.

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/HDAC/">HDAC</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"&gt; (View all antibody categories related to Transcription Regulators)</a>

Note

AntibodyPlus can customize Phospho-HDAC5-S498 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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