Rabbit Polyclonal Phospho-Histone H1 (T17) antibody (STJ91085)

$99.00$319.00

Reactivity: Human, Mouse, Monkey
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Histone H1 (T17) antibody (STJ91085)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

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Alternative names:

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SCBT cat No: sc-514856|

 

Name

Histone H1 (phospho Thr17) Polyclonal Antibody

Catalogue No.

STJ91085

Reactivity

Human, Mouse, Monkey

Specificity

Phospho-Histone H1 (T17) Polyclonal Antibody detects endogenous levels of Histone H1 protein only when phosphorylated at T17.

Immunogen

Synthesized phospho-peptide derived from Histone H1 (phospho Thr17) at AA range 1-80

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

31 kDa

Formulation

Histone H1 (phospho Thr17) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Histone H1 (phospho Thr17) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Histone H1.5 antibody, Histone H1a antibody, Histone H1b antibody, Histone H1s-3 antibody

Database links

Human UniProt/Swiss-Prot:P16401 ;Human Entrez Gene: 3009;

Protein names

Histone H1.5 , Histone H1a , Histone H1b , Histone H1s-3

Protein function

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Protein tissue specificity

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.

Protein sequence and domain

The C-terminal domain is required for high-affinity binding to chromatin. / Belongs to the histone H1/H5 family. / Contains 1 H15 (linker histone H1/H5 globular) domain.

Protein post-translational modifications

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter (By similarity). Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase. / Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

Protein cellular localization

Nucleus / Chromosome

Research area

All research areas>Transcription Regulators>Histone
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Histone H1 (phospho Thr17) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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