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Rabbit Polyclonal Phospho-HSF1-S303 antibody (AP0363)
Supplier: ABclonal Inc.
Recommended applications: WB,IHC
WB 1:500 – 1:2000
IHC 1:50 – 1:100
Recommended protocols: check protocols
Click or hover above images to see image description for Rabbit Polyclonal Phospho-HSF1-S303 antibody.
Check alternative names for the antibodyExpand
Heat shock factor 1 antibody|Heat shock factor protein 1 antibody|Heat shock transcription factor 1 antibody|HSF 1 antibody|hsf1 antibody|HSF1_HUMAN antibody|HSTF 1 antibody|HSTF1 antibody|Anti-HSF1 antibody – ChIP Grade (ab2923)
SCBT cat No: sc-13516|sc-393677|sc-8061|sc-17756|sc-393509|sc-17757|sc-9144|sc-13517|sc-8062|sc-377045|
Rabbit Polyclonal Phospho-HSF1-S303 antibody
|Catalogue No.|| |
A phospho specific peptide corresponding to residues surrounding S303 of human HSF1
|Recommended dilution|| |
WB 1:500 – 1:2000<br/>IHC 1:50 – 1:100
|Molecular weight|| |
Phospho-HSF1-S303 antibody was tube-contained.
Store at -20 Celsius degree. Avoid freeze / thaw cycles.
|Alternative antibody names|| |
|Database links|| |
Human UniProt/Swiss-Prot:<a href="http://www.uniprot.org/uniprot/Q00613">Q00613</a>
|Protein names|| |
|Protein function|| |
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
|Protein sequence and domain|| |
Belongs to the HSF family. the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
|Protein post-translational modifications|| |
Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.; Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
|Protein cellular localization|| |
Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.
|Research area|| |
<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/HSF/">HSF</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"> (View all antibody categories related to Transcription Regulators)</a>
AntibodyPlus can customize Phospho-HSF1-S303 antibody according to your requirement, including bulk product size,etc. Please contact email@example.com. AntibodyPlus provides antibody trial sample for your own antibody validation.
|Product type|| |
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