Rabbit Polyclonal Phospho-HSF1-S303 antibody (AP0363)

$99.00$259.00

Reactivity: Human
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-HSF1-S303 antibody (AP0363)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit Polyclonal Phospho-HSF1-S303 antibody.

Alternative names:

Check alternative names for the antibody

Expand

HSTF1 antibody;
Heat shock factor 1 antibody|Heat shock factor protein 1 antibody|Heat shock transcription factor 1 antibody|HSF 1 antibody|hsf1 antibody|HSF1_HUMAN antibody|HSTF 1 antibody|HSTF1 antibody|Anti-HSF1 antibody – ChIP Grade (ab2923)

SCBT cat No: sc-13516|sc-393677|sc-8061|sc-17756|sc-393509|sc-17757|sc-9144|sc-13517|sc-8062|sc-377045|

Name

Rabbit Polyclonal Phospho-HSF1-S303 antibody

Catalogue No.

AP0363

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S303 of human HSF1

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

82kDa

Formulation

Phospho-HSF1-S303 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

HSTF1 antibody;

Protein names

HSTF1;

Protein function

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Protein sequence and domain

Belongs to the HSF family. the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

Protein post-translational modifications

Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.; Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

Protein cellular localization

Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.

Background

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Transcription-Regulators/">Transcription Regulators</a>><a href="http://antibodyplus.com/product-tag/HSF/">HSF</a><br><a href="http://antibodyplus.com/product-tag/Transcription-Regulators/"&gt; (View all antibody categories related to Transcription Regulators)</a>

Note

AntibodyPlus can customize Phospho-HSF1-S303 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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