Rabbit Polyclonal Phospho-HSF1 (S303) antibody (STJ90293)


Reactivity: Human, Mouse
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-HSF1 (S303) antibody (STJ90293)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for HSF1 (phospho Ser303) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


HSF1 antibody, HSTF1 antibody,|Heat shock factor 1 antibody|Heat shock factor protein 1 antibody|Heat shock transcription factor 1 antibody|HSF 1 antibody|hsf1 antibody|HSF1_HUMAN antibody|HSTF 1 antibody|HSTF1 antibody|Anti-HSF1 antibody – ChIP Grade (ab2923)
SCBT cat No: sc-13516|sc-393677|sc-8061|sc-17756|sc-393509|sc-17757|sc-9144|sc-13517|sc-8062|sc-377045|



HSF1 (phospho Ser303) Polyclonal Antibody

Catalogue No.



Human, Mouse


Phospho-HSF1 (S303) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S303.


Synthesized phospho-peptide derived from HSF1 (phospho Ser303) at AA range 240-320





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;







Molecular weight

82/76 kDa


HSF1 (phospho Ser303) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


HSF1 (phospho Ser303) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Heat shock factor protein 1 antibody, HSF 1 antibody, Heat shock transcription factor 1 antibody, HSTF 1 antibody

Database links

Human UniProt/Swiss-Prot:Q00613;Mouse UniPort/Swiss-Prot: P38532;Rat UniProt/Swiss-Port: F1MAF1;Human Entrez Gene: 3297;Mouse Entrez Gene: 15499;Rat Entrez Gene: Rn.20418

Protein names

Heat shock factor protein 1 , HSF 1 , Heat shock transcription factor 1 , HSTF 1

Protein function

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Protein sequence and domain

the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. / Belongs to the HSF family.

Protein post-translational modifications

Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity. / Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Transcription Regulators>HSF
(View all antibody categories related to Transcription Regulators)


AntibodyPlus can customize HSF1 (phospho Ser303) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.


St John’s Laboratory Ltd.

Product type

Primary antibody


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