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Rabbit Polyclonal Phospho-HSF1 (S303) antibody (STJ90293)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, IF, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;
Recommended protocols: check protocols
Click or hover above images to see image description for HSF1 (phospho Ser303) Polyclonal Antibody.
Check alternative names for the antibodyExpand
HSF1 antibody, HSTF1 antibody,|Heat shock factor 1 antibody|Heat shock factor protein 1 antibody|Heat shock transcription factor 1 antibody|HSF 1 antibody|hsf1 antibody|HSF1_HUMAN antibody|HSTF 1 antibody|HSTF1 antibody|Anti-HSF1 antibody – ChIP Grade (ab2923)
SCBT cat No: sc-13516|sc-393677|sc-8061|sc-17756|sc-393509|sc-17757|sc-9144|sc-13517|sc-8062|sc-377045|
HSF1 (phospho Ser303) Polyclonal Antibody
|Catalogue No.|| |
Phospho-HSF1 (S303) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S303.
Synthesized phospho-peptide derived from HSF1 (phospho Ser303) at AA range 240-320
WB, IHC, IF, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000;
|Molecular weight|| |
HSF1 (phospho Ser303) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
HSF1 (phospho Ser303) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Heat shock factor protein 1 antibody, HSF 1 antibody, Heat shock transcription factor 1 antibody, HSTF 1 antibody
|Protein names|| |
Heat shock factor protein 1 , HSF 1 , Heat shock transcription factor 1 , HSTF 1
|Protein function|| |
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
|Protein sequence and domain|| |
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. / Belongs to the HSF family.
|Protein post-translational modifications|| |
Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity. / Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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