Rabbit Polyclonal Phospho-HSP27 (S82) antibody (STJ90296)


Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-HSP27 (S82) antibody (STJ90296)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for HSP27 (phospho Ser82) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


HSPB1 antibody, HSP27 antibody, HSP28 antibody,|Heat shock 27kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|epididymis secretory protein Li 102 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27kDa protein 1 antibody|Heat shock 28kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|heat shock protein family B (small) member 1 antibody|HEL-S-102 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|HSP 27 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody|Anti-Hsp27 antibody [G3.1] (ab2790)
SCBT cat No: sc-1048|sc-13132|sc-59562|sc-9012|sc-51956|sc-51958|sc-1049|sc-135943|sc-398766|sc-1800|sc-28845|sc-1801|sc-376544|sc-47051|sc-135152|



HSP27 (phospho Ser82) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-HSP27 (S82) Polyclonal Antibody detects endogenous levels of HSP27 protein only when phosphorylated at S82.


Synthesized phospho-peptide derived from HSP27 (phospho Ser82) at AA range 20-100





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;







Molecular weight

27 kDa


HSP27 (phospho Ser82) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


HSP27 (phospho Ser82) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Heat shock protein beta-1 antibody, HspB1 antibody, 28 kDa heat shock protein antibody, Estrogen-regulated 24 kDa protein antibody, Heat shock 27 kDa protein antibody, HSP 27 antibody, Stress-responsive protein 27 antibody, SRP27 antibody

Database links

Human UniProt/Swiss-Prot:P04792;Mouse UniPort/Swiss-Prot: P14602;Rat UniProt/Swiss-Port: G3V913;Human Entrez Gene: 3315;Mouse Entrez Gene: 15507;Rat Entrez Gene: Rn.3841

Protein names

Heat shock protein beta-1 , HspB1 , 28 kDa heat shock protein , Estrogen-regulated 24 kDa protein , Heat shock 27 kDa protein , HSP 27 , Stress-responsive protein 27 , SRP27

Protein function

Involved in stress resistance and actin organization.

Protein tissue specificity

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

Involvement in disease

Charcot-Marie-Tooth disease 2F (CMT2F) [MIM:606595]: A dominant axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies (designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal degeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Onset of Charcot-Marie-Tooth disease type 2F is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. . Note: The disease is caused by mutations affecting the gene represented in this entry.; Neuronopathy, distal hereditary motor, 2B (HMN2B) [MIM:608634]: A neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the small heat shock protein (HSP20) family.

Protein post-translational modifications

Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock. / Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress leads to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

Protein cellular localization

Cytoplasm / Nucleus / Cytoplasm > cytoskeleton > spindle

Research area

All research areas>Signaling Intermediates>HSP
(View all antibody categories related to Signaling Intermediates)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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