Rabbit polyclonal Phospho-HSPB1-S82 antibody (AP0041)

$69.00$259.00

Reactivity: Human,Rat
Applications: WB,IHC,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-HSPB1-S82 antibody (AP0041)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC,IF

Recommended dilution:

WB 1:500 – 1:2000 IHC 1:50 – 1:200 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-HSPB1-S82 antibody.

Alternative names:

Check alternative names for the antibody

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CMT2F antibody, HMN2B antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, HS.76067 antibody, DKFZp586P1322 antibody
Heat shock 27kDa protein antibody|28 kDa heat shock protein antibody|CMT2F antibody|DKFZp586P1322 antibody|epididymis secretory protein Li 102 antibody|Estrogen regulated 24 kDa protein antibody|Estrogen-regulated 24 kDa protein antibody|Heat shock 25kDa protein 1 antibody|Heat shock 27 kDa protein antibody|Heat shock 27kD protein 1 antibody|Heat shock 27kDa protein 1 antibody|Heat shock 28kDa protein 1 antibody|Heat Shock Protein 27 antibody|Heat shock protein beta 1 antibody|Heat shock protein beta-1 antibody|heat shock protein family B (small) member 1 antibody|HEL-S-102 antibody|HMN2B antibody|HS.76067 antibody|Hsp 25 antibody|HSP 27 antibody|Hsp 28 antibody|Hsp B1 antibody|Hsp25 antibody|HSP27 antibody|Hsp28 antibody|HspB1 antibody|HSPB1_HUMAN antibody|SRP27 antibody|Stress responsive protein 27 antibody|Stress-responsive protein 27 antibody|Anti-Hsp27 antibody [G3.1] (ab2790)

SCBT cat No: sc-1048|sc-13132|sc-59562|sc-9012|sc-51956|sc-51958|sc-1049|sc-135943|sc-398766|sc-1800|sc-28845|sc-1801|sc-376544|sc-47051|sc-135152|

Name

Rabbit polyclonal Phospho-HSPB1-S82 antibody

Catalogue No.

AP0041

Reactivity

Human, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding S82 of human HSPB1

Host

Rabbit

Applications

WB, IHC, IF

Recommended dilution

WB 1:500 – 1:2000
IHC 1:50 – 1:200
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: /Observed: Refer to Figures

Formulation

Phospho-HSPB1-S82 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-HSPB1-S82 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

CMT2F antibody, HMN2B antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, HS.76067 antibody, DKFZp586P1322 antibody

Database links

Human UniProt/Swiss-Prot:P04792

Protein names

CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, DKFZp586P1322

Protein function

Involved in stress resistance and actin organization.

Protein tissue specificity

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

Protein sequence and domain

Belongs to the small heat shock protein (HSP20) family.

Protein post-translational modifications

Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.; Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress leads to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.

Protein cellular localization

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Background

Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

Research area

All research areas>Signaling Intermediates>HSP
(View all antibody categories related to Signaling Intermediates)

Note

AntibodyPlus can customize Phospho-HSPB1-S82 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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