Rabbit Polyclonal Phospho-IkappaB-alpha (Y42) antibody (STJ90312)


Reactivity: Human, Mouse, Rat
Applications: WB, IHC, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-IkappaB-alpha (Y42) antibody (STJ90312)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for IkappaB-alpha (phospho Tyr42) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody


NFKBIA antibody, IKBA antibody, MAD3 antibody, NFKBI antibody,|I kappa B alpha antibody|I-kappa-B-alpha antibody|IkappaBalpha antibody|IkB-alpha antibody|IKBA antibody|IKBA_HUMAN antibody|IKBalpha antibody|MAD 3 antibody|MAD3 antibody|Major histocompatibility complex enhancer-binding protein MAD3 antibody|NF kappa B inhibitor alpha antibody|NF-kappa-B inhibitor alpha antibody|NFKBI antibody|NFKBIA antibody|Nuclear factor of kappa light chain gene enhancer in B cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody|Anti-IKB alpha antibody [E130] (ab32518)
SCBT cat No: sc-71291|sc-52900|sc-23459|sc-23460|sc-25105|sc-71525|sc-52936|sc-23461|sc-56196|sc-56217|sc-52953|sc-71965|



IkappaB-alpha (phospho Tyr42) Polyclonal Antibody

Catalogue No.



Human, Mouse, Rat


Phospho-IkappaB-alpha (Y42) Polyclonal Antibody detects endogenous levels of IkappaB-alpha protein only when phosphorylated at Y42.


Synthesized phospho-peptide derived from IkappaB-alpha (phospho Tyr42) at AA range 20-100





Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;







Molecular weight

39 kDa


IkappaB-alpha (phospho Tyr42) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.


1 mg/ml


IkappaB-alpha (phospho Tyr42) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.


-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

NF-kappa-B inhibitor alpha antibody, I-kappa-B-alpha antibody, IkB-alpha antibody, IkappaBalpha antibody, Major histocompatibility complex enhancer-binding protein MAD3 antibody

Database links

Human UniProt/Swiss-Prot:P25963;Mouse UniPort/Swiss-Prot: Q9Z1E3;Rat UniProt/Swiss-Port: Q63746;Human Entrez Gene: 4792;Mouse Entrez Gene: 18035;Rat Entrez Gene: Rn.12550

Protein names

NF-kappa-B inhibitor alpha , I-kappa-B-alpha , IkB-alpha , IkappaBalpha , Major histocompatibility complex enhancer-binding protein MAD3

Protein function

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Protein tissue specificity

Induced in adherent monocytes.

Involvement in disease

Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID) [MIM:612132]: A form of ectoderma dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. This form of ectodermal dysplasia is associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection. . Note: The disease is caused by mutations affecting the gene represented in this entry.

Protein sequence and domain

Belongs to the NF-kappa-B inhibitor family. / Contains 5 ANK repeats.

Protein post-translational modifications

Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. / Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. / Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. / Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

Protein cellular localization

Cytoplasm / Nucleus

Research area

All research areas>Transcription Regulators>I κ B
(View all antibody categories related to Transcription Regulators)


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St John’s Laboratory Ltd.

Product type

Primary antibody


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