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Rabbit Polyclonal Phospho-IKKalpha/beta (S176/177) antibody (STJ90301)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
Recommended protocols: check protocols
Click or hover above images to see image description for IKKalpha/beta (phospho Ser176/177) Polyclonal Antibody.
Check alternative names for the antibodyExpand
CHUK antibody, IKKA antibody, TCF16 antibody,|chuk antibody|CHUK1 antibody|Conserved Helix Loop Helix Ubiquitous Kinase antibody|Conserved helix loop ubiquitous kinase antibody|Conserved helix-loop-helix ubiquitous kinase antibody|I Kappa B Kinase 1 antibody|I Kappa B Kinase Alpha antibody|I-kappa-B kinase 1 antibody|I-kappa-B kinase alpha antibody|IkappaB kinase antibody|IkB kinase alpha subunit antibody|IkBKA antibody|IKK 1 antibody|IKK A antibody|IKK a kinase antibody|IKK-A antibody|IKK-alpha antibody|IKK1 antibody|IKKA antibody|IKKA_HUMAN antibody|Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody|Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody|NFKBIKA antibody|Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody|Nuclear factor NF kappa B inhibitor kinase alpha antibody|Nuclear factor NF-kappa-B inhibitor kinase alpha antibody|Nuclear factor NFkappaB inhibitor kinase alpha antibody|Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody|TCF-16 antibody|TCF16 antibody|Transcription factor 16 antibody|Anti-IKK alpha antibody [Y463] (ab32041)
SCBT cat No: sc-7190|
IKKalpha/beta (phospho Ser176/177) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-IKKalpha/beta (S176/177) Polyclonal Antibody detects endogenous levels of IKKalpha/beta protein only when phosphorylated at S176/177.
Synthesized phospho-peptide derived from IKKalpha/beta (phospho Ser176/177) at AA range 120-200
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000;
|Molecular weight|| |
IKKalpha/beta (phospho Ser176/177) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
IKKalpha/beta (phospho Ser176/177) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody, I-kappa-B kinase alpha antibody, IKK-A antibody, IKK-alpha antibody, IkBKA antibody, IkappaB kinase antibody, Conserved helix-loop-helix ubiquitous kinase antibody, I-kappa-B kinase 1 antibody, IKK1 antibody, Nuclear factor NF-kappa-B inhibitor kinase alpha antibody, NFKBIKA antibody, Transcription factor 16 antibody, TCF-16 antibody
|Protein names|| |
Inhibitor of nuclear factor kappa-B kinase subunit alpha , I-kappa-B kinase alpha , IKK-A , IKK-alpha , IkBKA , IkappaB kinase , Conserved helix-loop-helix ubiquitous kinase , I-kappa-B kinase 1 , IKK1 , Nuclear factor NF-kappa-B inhibitor kinase alpha , NFKBIKA , Transcription factor 16 , TCF-16
|Protein function|| |
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non-canonical NF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at ‘Ser-10’ that are subsequently acetylated at ‘Lys-14’ by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3. Also phosphorylates FOXO3 and may regulate this pro-apoptotic transcription factor . / ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]. / Activated when phosphorylated and inactivated when dephosphorylated.
|Protein tissue specificity|| |
|Involvement in disease|| |
Cocoon syndrome (COCOS) [MIM:613630]: A lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG. / Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. / (Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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