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Rabbit Polyclonal Phospho-IKKbeta (Y199) antibody (STJ90814)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
Recommended protocols: check protocols
Click or hover above images to see image description for IKKbeta (phospho Tyr199) Polyclonal Antibody.
Check alternative names for the antibodyExpand
IKBKB antibody, IKKB antibody,|I kappa B kinase 2 antibody|I kappa B kinase beta antibody|I-kappa-B kinase 2 antibody|I-kappa-B-kinase beta antibody|IkBKB antibody|IKK beta antibody|IKK-B antibody|IKK-beta antibody|IKK2 antibody|IKKB antibody|IKKB_HUMAN antibody|IMD15 antibody|Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody|Inhibitor of nuclear factor kappa-B kinase subunit beta antibody|NFKBIKB antibody|Nuclear factor NF-kappa-B inhibitor kinase beta antibody|Anti-IKK beta antibody [EPR6043] (ab124957)
SCBT cat No: sc-7607|sc-56918|sc-135948|sc-130152|sc-7329|sc-271782|sc-8014|sc-34673|sc-34674|sc-7330|sc-71331|sc-52930|sc-56919|
IKKbeta (phospho Tyr199) Polyclonal Antibody
|Catalogue No.|| |
Human, Mouse, Rat
Phospho-IKKbeta (Y199) Polyclonal Antibody detects endogenous levels of IKKbeta protein only when phosphorylated at Y199.
Synthesized phospho-peptide derived from IKKbeta (phospho Tyr199) at AA range 140-220
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000;
|Molecular weight|| |
IKKbeta (phospho Tyr199) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
IKKbeta (phospho Tyr199) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Inhibitor of nuclear factor kappa-B kinase subunit beta antibody, I-kappa-B-kinase beta antibody, IKK-B antibody, IKK-beta antibody, IkBKB antibody, I-kappa-B kinase 2 antibody, IKK2 antibody, Nuclear factor NF-kappa-B inhibitor kinase beta antibody, NFKBIKB antibody
|Protein names|| |
Inhibitor of nuclear factor kappa-B kinase subunit beta , I-kappa-B-kinase beta , IKK-B , IKK-beta , IkBKB , I-kappa-B kinase 2 , IKK2 , Nuclear factor NF-kappa-B inhibitor kinase beta , NFKBIKB
|Protein function|| |
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. / ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
|Protein tissue specificity|| |
Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
|Involvement in disease|| |
Immunodeficiency 15 (IMD15) [MIM:615592]: An autosomal recessive primary immunodeficiency disorder characterized by onset in infancy of life-threatening bacterial, fungal, and viral infections and failure to thrive. Laboratory studies show hypo- or agammaglobulinemia with relatively normal numbers of B and T-cells, and impaired differentiation and activation of immune cells. . Note: The disease is caused by mutations affecting the gene represented in this entry.
|Protein sequence and domain|| |
The kinase domain is located in the N-terminal region. The leucine zipper is important to allow homo- and hetero-dimerization. At the C-terminal region is located the region responsible for the interaction with NEMO/IKBKG. / Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK as well as TBK1 and PRKCZ; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. Phosphorylated by the IKK-related kinases TBK1 and IKBKE, which is associated with reduced CHUK/IKKA and IKBKB activity and NF-kappa-B-dependent gene transcription. Dephosphorylated at Ser-177 and Ser-181 by PPM1A and PPM1B. / (Microbial infection) Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway. / Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, ‘Ser-163’ does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on ‘Ser-163’ modulates phosphorylation on C-terminal serine residues. / (Microbial infection) Monoubiquitination by TRIM21 is disrupted by Yersinia yopJ. / Hydroxylated by PHD1/EGLN2, loss of hydroxylation under hypoxic conditions results in activation of NF-kappa-B.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Membrane raft
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St John’s Laboratory Ltd.
|Product type|| |
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