Rabbit Polyclonal Phospho-IRAK-1 (T209) antibody (STJ90531)

$99.00$319.00

Reactivity: Human
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-IRAK-1 (T209) antibody (STJ90531)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:40000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for IRAK-1 (phospho Thr209) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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IRAK1 antibody, IRAK antibody,|Il1rak antibody|Interleukin 1 receptor associated kinase 1 antibody|Interleukin-1 receptor-associated kinase 1 antibody|IRAK antibody|IRAK-1 antibody|Irak1 antibody|IRAK1_HUMAN antibody|mPLK antibody|OTTHUMP00000026014 antibody|OTTHUMP00000026015 antibody|OTTHUMP00000026020 antibody|OTTHUMP00000180621 antibody|Pelle antibody|Pelle homolog antibody|Pelle-like protein kinase antibody|Plpk antibody|Anti-IRAK antibody (ab238)
SCBT cat No: sc-55530|sc-1894|sc-5287|sc-515512|sc-5288|sc-7883|sc-23652|

 

Name

IRAK-1 (phospho Thr209) Polyclonal Antibody

Catalogue No.

STJ90531

Reactivity

Human

Specificity

Phospho-IRAK-1 (T209) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T209.

Immunogen

Synthesized phospho-peptide derived from IRAK-1 (phospho Thr209) at AA range 150-230

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:40000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

77 kDa

Formulation

IRAK-1 (phospho Thr209) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

IRAK-1 (phospho Thr209) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Interleukin-1 receptor-associated kinase 1 antibody, IRAK-1 antibody,

Database links

Human UniProt/Swiss-Prot:P51617;Mouse UniPort/Swiss-Prot: Q62406;Rat UniProt/Swiss-Port: B2RYH5;Human Entrez Gene: 3654;Mouse Entrez Gene: 16179;Rat Entrez Gene: Rn.22238

Protein names

Interleukin-1 receptor-associated kinase 1 , IRAK-1 ,

Protein function

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. / ATP + a protein = ADP + a phosphoprotein. / Mg2+

Protein tissue specificity

Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein sequence and domain

The ProST region is composed of many proline and serine residues (more than 20 of each) and some threonines. This region is the site of IRAK-1 hyperphosphorylation. / Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. / Contains 1 death domain. / Contains 1 protein kinase domain.

Protein post-translational modifications

Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity. / Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through ‘Lys-63’. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation.

Protein cellular localization

Cytoplasm / Nucleus / Lipid droplet

Research area

All research areas>Kinases and Phosphatases>IRAK
(View all antibody categories related to Kinases and Phosphatases)

Note

AntibodyPlus can customize IRAK-1 (phospho Thr209) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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