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Rabbit Polyclonal Phospho-IRAK-1 (T209) antibody (STJ90531)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, ELISA
Recommended dilution: WB 1:500-1:2000; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for IRAK-1 (phospho Thr209) Polyclonal Antibody.
Check alternative names for the antibodyExpand
IRAK1 antibody, IRAK antibody,|Il1rak antibody|Interleukin 1 receptor associated kinase 1 antibody|Interleukin-1 receptor-associated kinase 1 antibody|IRAK antibody|IRAK-1 antibody|Irak1 antibody|IRAK1_HUMAN antibody|mPLK antibody|OTTHUMP00000026014 antibody|OTTHUMP00000026015 antibody|OTTHUMP00000026020 antibody|OTTHUMP00000180621 antibody|Pelle antibody|Pelle homolog antibody|Pelle-like protein kinase antibody|Plpk antibody|Anti-IRAK antibody (ab238)
SCBT cat No: sc-55530|sc-1894|sc-5287|sc-515512|sc-5288|sc-7883|sc-23652|
IRAK-1 (phospho Thr209) Polyclonal Antibody
|Catalogue No.|| |
Phospho-IRAK-1 (T209) Polyclonal Antibody detects endogenous levels of IRAK-1 protein only when phosphorylated at T209.
Synthesized phospho-peptide derived from IRAK-1 (phospho Thr209) at AA range 150-230
|Recommended dilution|| |
WB 1:500-1:2000; ELISA 1:40000;
|Molecular weight|| |
IRAK-1 (phospho Thr209) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
IRAK-1 (phospho Thr209) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Interleukin-1 receptor-associated kinase 1 antibody, IRAK-1 antibody,
|Protein names|| |
Interleukin-1 receptor-associated kinase 1 , IRAK-1 ,
|Protein function|| |
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. / ATP + a protein = ADP + a phosphoprotein. / Mg2+
|Protein tissue specificity|| |
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
|Protein sequence and domain|| |
The ProST region is composed of many proline and serine residues (more than 20 of each) and some threonines. This region is the site of IRAK-1 hyperphosphorylation. / Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. / Contains 1 death domain. / Contains 1 protein kinase domain.
|Protein post-translational modifications|| |
Following recruitment on the activated receptor complex, phosphorylated on Thr-209, probably by IRAK4, resulting in a conformational change of the kinase domain, allowing further phosphorylations to take place. Thr-387 phosphorylation in the activation loop is required to achieve full enzymatic activity. / Polyubiquitinated by TRAF6 after cell stimulation with IL-1-beta by PELI1, PELI2 and PELI3. Polyubiquitination occurs with polyubiquitin chains linked through ‘Lys-63’. Ubiquitination promotes interaction with NEMO/IKBKG. Also sumoylated; leading to nuclear translocation.
|Protein cellular localization|| |
Cytoplasm / Nucleus / Lipid droplet
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St John’s Laboratory Ltd.
|Product type|| |
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