Rabbit polyclonal Phospho-Jun-S63 antibody (AP0048)

$69.00$259.00

Reactivity: Human
Applications: WB,IF
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit polyclonal Phospho-Jun-S63 antibody (AP0048)

Supplier: ABclonal Inc.

Recommended applications: WB,IF

Recommended dilution:

WB 1:500 – 1:2000 IF 1:50 – 1:200

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Rabbit polyclonal Phospho-Jun-S63 antibody.

Alternative names:

Check alternative names for the antibody

Expand

AP1 antibody, AP-1 antibody, c-Jun antibody, JUN antibody
Activator protein 1 antibody|AP 1 antibody|DKFZp686C0818 antibody|FBJ murine osteosarcoma viral oncogene homolog B antibody|fosB antibody|FOSB_HUMAN antibody|G0/G1 switch regulatory protein 3 antibody|G0S3 antibody|GOS 3 antibody|GOS B antibody|GOS3 antibody|GOSB antibody|MGC42291 antibody|Oncogene FOS B antibody|Oncogene FOSB antibody|Protein fos B antibody|Protein fosB antibody|Anti-c-Jun antibody – ChIP Grade

SCBT cat No: sc-166540|sc-376488|sc-44|sc-74543|sc-1694|sc-45|sc-162685|sc-398286|sc-393629|sc-393544|sc-167469|sc-240235|sc-374394|sc-376355|

Name

Rabbit polyclonal Phospho-Jun-S63 antibody

Catalogue No.

AP0048

Reactivity

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S63 of human c-Jun

Host

Rabbit

Applications

WB, IF

Recommended dilution

WB 1:500 – 1:2000
IF 1:50 – 1:200

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

Predicted: /Observed: Refer to Figures

Formulation

Phospho-Jun-S63 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Phospho-Jun-S63 antibody was purified using affinity purification.

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

AP1 antibody, AP-1 antibody, c-Jun antibody, JUN antibody

Database links

Human UniProt/Swiss-Prot:P05412

Protein names

AP1, AP-1, c-Jun, JUN

Protein function

Transcription factor that recognizes and binds to the enhancer heptamer motif 5′-TGA[CG]TCA-3′. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells . Binds to the USP28 promoter in colorectal cancer (CRC) cells .

Protein sequence and domain

Belongs to the bZIP family. Jun subfamily.; Contains 1 bZIP (basic-leucine zipper) domain.

Protein post-translational modifications

Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.; Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.; Acetylated at Lys-271 by EP300.

Protein cellular localization

Nucleus.

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (reviewed in 1). Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6). AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11,12).

Research area

All research areas>Transcription Regulators>Jun
(View all antibody categories related to Transcription Regulators)

Note

AntibodyPlus can customize Phospho-Jun-S63 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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