Rabbit Polyclonal Phospho-KIT-Y936 antibody (AP0286)

$99.00$259.00

Reactivity: Human,Rat
Applications: WB,IHC
Conjugation: Unconjugated
Supplier: ABclonal Inc.

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Rabbit Polyclonal Phospho-KIT-Y936 antibody (AP0286)

Supplier: ABclonal Inc.

Recommended applications: WB,IHC

Recommended dilution:

WB 1:500 – 1:2000
IHC 1:50 – 1:100

Recommended protocols: check protocols

Image descriptions:

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Alternative names:

Check alternative names for the antibody

Expand

PBT antibody; SCFR antibody; C-Kit antibody; CD117 antibody;
C Kit antibody|c-Kit antibody|c-Kit Ligand antibody|CD117 antibody|Kit antibody|Kit Ligand antibody|KIT oncogene antibody|KIT proto oncogene receptor tyrosine kinase antibody|KIT_HUMAN antibody|Mast cell growth factor receptor antibody|Mast/stem cell growth factor receptor Kit antibody|MGF antibody|p145 c-kit antibody|PBT antibody|Piebald trait protein antibody|Proto oncogene c Kit antibody|Proto oncogene tyrosine protein kinase Kit antibody|Proto-oncogene c-Kit antibody|SCF Receptor antibody|SCFR antibody|soluble KIT variant 1 antibody|Steel Factor Receptor antibody|Stem cell factor receptor antibody|tyrosine protein kinase Kit antibody|Tyrosine-protein kinase Kit antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody|v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody|v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody|Anti-c-Kit antibody [YR145] (ab32363)

SCBT cat No: sc-19983|sc-19619|sc-53147|sc-70455|sc-13508|sc-1493|sc-168|sc-17806|sc-365504|sc-393910|sc-5535|sc-1494|

Name

Rabbit Polyclonal Phospho-KIT-Y936 antibody

Catalogue No.

AP0286

Reactivity

Human, Rat

Immunogen

A phospho specific peptide corresponding to residues surrounding Y936 of human KIT

Host

Rabbit

Applications

WB, IHC

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

120kD 145kD

Formulation

Phospho-KIT-Y936 antibody was tube-contained.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification

Affinity purification

Storage

Store at -20 Celsius degree. Avoid freeze / thaw cycles.

Alternative antibody names

PBT antibody; SCFR antibody; C-Kit antibody; CD117 antibody;

Protein names

PBT; SCFR; C-Kit; CD117;

Protein function

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Protein tissue specificity

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Protein sequence and domain

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.; Contains 5 Ig-like C2-type (immunoglobulin-like) domains.; Contains 1 protein kinase domain.

Protein post-translational modifications

Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.; Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

Protein cellular localization

Isoform 1: Cell membrane; Single-pass type I membrane protein.; Isoform 2: Cell membrane; Single-pass type I membrane protein.; Isoform 3: Cytoplasm. Note=Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.

Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Research area

<a href="http://antibodyplus.com/research-areas/">All research areas</a>><a href="http://antibodyplus.com/Membrane-Receptors/">Membrane Receptors</a>><a href="http://antibodyplus.com/product-tag/c-Kit/">c-Kit</a><br><a href="http://antibodyplus.com/product-tag/Membrane-Receptors/"&gt; (View all antibody categories related to Membrane Receptors)</a>

Note

AntibodyPlus can customize Phospho-KIT-Y936 antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provides antibody trial sample for your own antibody validation.

Product type

Primary antibody

Supplier

ABclonal Inc.

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