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Rabbit Polyclonal Phospho-Krs-1/2 (T183) antibody (STJ90855)
Supplier: St John’s Laboratory Ltd.
Recommended applications: WB, IHC, ELISA
Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;
Recommended protocols: check protocols
Click or hover above images to see image description for Krs-1/2 (phospho Thr183) Polyclonal Antibody.
Check alternative names for the antibodyExpand
STK3 antibody, KRS1 antibody, MST2 antibody,|0610042I06Rik antibody|MST3 antibody|YSK1 antibody|Anti-STK3 antibody [EP1466Y] (ab52641)
SCBT cat No: sc-130405|
Krs-1/2 (phospho Thr183) Polyclonal Antibody
|Catalogue No.|| |
Phospho-Krs-1/2 (T183) Polyclonal Antibody detects endogenous levels of Krs-1/2 protein only when phosphorylated at T183.
Synthesized phospho-peptide derived from Krs-1/2 (phospho Thr183) at AA range 120-200
WB, IHC, ELISA
|Recommended dilution|| |
WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000;
|Molecular weight|| |
Krs-1/2 (phospho Thr183) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Krs-1/2 (phospho Thr183) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-20 Celsius degree. Avoid repeated freeze/thaw cycles.
|Alternative antibody names|| |
Serine/threonine-protein kinase 3 antibody, Mammalian STE20-like protein kinase 2 antibody, MST-2 antibody, STE20-like kinase MST2 antibody, Serine/threonine-protein kinase Krs-1 antibody
|Protein names|| |
Serine/threonine-protein kinase 3 , Mammalian STE20-like protein kinase 2 , MST-2 , STE20-like kinase MST2 , Serine/threonine-protein kinase Krs-1
|Protein function|| |
Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on ‘Ser-259’. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on ‘Thr-74’. Acts cooperatively with MOBKL1B to activate STK38. / ATP + a protein = ADP + a phosphoprotein. / Mg2+ / Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene product RAF1. Activated by RASSF1 which acts by preventing its dephosphorylation.
|Protein tissue specificity|| |
Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
|Protein sequence and domain|| |
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. / Contains 1 protein kinase domain. / Contains 1 SARAH domain.
|Protein post-translational modifications|| |
Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1. / Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).
|Protein cellular localization|| |
Cytoplasm / Nucleus
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St John’s Laboratory Ltd.
|Product type|| |
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