Rabbit Polyclonal Phospho-Ku-80 (T714) antibody (STJ91033)

$99.00$319.00

Reactivity: Human, Monkey
Applications: WB, IHC, IF, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-Ku-80 (T714) antibody (STJ91033)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, IHC, IF, ELISA

Recommended dilution: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for Ku-80 (phospho Thr714) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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XRCC5 antibody, G22P2 antibody,|86 kDa subunit of Ku antigen antibody|KUB 2 antibody|KUB2 antibody|Lupus Ku autoantigen protein p86 antibody|NFIV antibody|Nuclear factor IV antibody|Thyroid lupus autoantigen antibody|Thyroid-lupus autoantigen antibody|TLAA antibody|X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody|X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody|X-ray repair cross-complementing protein 5 antibody|Xray repair complementing defective repair in Chinese hamster cells 5 antibody|XRCC 5 antibody|XRCC5 antibody|XRCC5_HUMAN antibody|Anti-Ku80 antibody [5C5] (ab119935)
SCBT cat No: sc-53021|sc-135964|sc-5280|sc-1484|sc-9034|sc-33653|sc-1485|sc-56135|sc-56136|

 

Name

Ku-80 (phospho Thr714) Polyclonal Antibody

Catalogue No.

STJ91033

Reactivity

Human, Monkey

Specificity

Phospho-Ku-80 (T714) Polyclonal Antibody detects endogenous levels of Ku-80 protein only when phosphorylated at T714.

Immunogen

Synthesized phospho-peptide derived from Ku-80 (phospho Thr714) at AA range 660-740

Host

Rabbit

Applications

WB, IHC, IF, ELISA

Recommended dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

83 kDa

Formulation

Ku-80 (phospho Thr714) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

Ku-80 (phospho Thr714) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

X-ray repair cross-complementing protein 5 antibody, 86 kDa subunit of Ku antigen antibody, ATP-dependent DNA helicase 2 subunit 2 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC box-binding factor 85 kDa subunit antibody, CTC85 antibody, CTCBF antibody, DNA repair protein XRCC5 antibody, Ku80 antibody, Ku86 antibody, Lupus Ku autoantigen protein p86 antibody, Nuclear factor IV antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, double-strand-break rejoining antibody

Database links

Human UniProt/Swiss-Prot:P13010;Mouse UniPort/Swiss-Prot: P27641;Rat UniProt/Swiss-Port: Q6P7P8;Human Entrez Gene: 7520;Mouse Entrez Gene: 22596;Rat Entrez Gene: Rn.52078

Protein names

X-ray repair cross-complementing protein 5 , 86 kDa subunit of Ku antigen , ATP-dependent DNA helicase 2 subunit 2 , ATP-dependent DNA helicase II 80 kDa subunit , CTC box-binding factor 85 kDa subunit , CTC85 , CTCBF , DNA repair protein XRCC5 , Ku80 , Ku86 , Lupus Ku autoantigen protein p86 , Nuclear factor IV , Thyroid-lupus autoantigen , TLAA , X-ray repair complementing defective repair in Chinese hamster cells 5 , double-strand-break rejoining

Protein function

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3′-5′ direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together . The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression . The XRCC5/6 dimer probably also acts as a 5′-deoxyribose-5-phosphate lyase (5′-dRP lyase), by catalyzing the beta-elimination of the 5′ deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5′-dRP activity, and allows to ‘clean’ the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription .

Protein tissue specificity

Expression increases during promyelocyte differentiation.

Protein sequence and domain

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. / Belongs to the ku80 family. / Contains 1 Ku domain.

Protein post-translational modifications

Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity. / Sumoylated. / Ubiquitinated by RNF8 via ‘Lys-48’-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites . Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks .

Protein cellular localization

Nucleus / Nucleus > nucleolus / Chromosome

Research area

All research areas>Tumor Suppressor/Apoptosis>Ku Lupus Antigen
(View all antibody categories related to Tumor Suppressor/Apoptosis)

Note

AntibodyPlus can customize Ku-80 (phospho Thr714) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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