Rabbit Polyclonal Phospho-LEF-1 (S42) antibody (STJ90534)

$99.00$319.00

Reactivity: Human, Mouse, Rat
Applications: WB, ELISA
Conjugation: Unconjugated
Supplier: St John’s Laboratory Ltd.

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Rabbit Polyclonal Phospho-LEF-1 (S42) antibody (STJ90534)

Supplier: St John’s Laboratory Ltd.

Recommended applications: WB, ELISA

Recommended dilution: WB 1:500-1:2000; ELISA 1:5000;

Recommended protocols: check protocols

Image descriptions:

Click or hover above images to see image description for LEF-1 (phospho Ser42) Polyclonal Antibody.

Alternative names:

Check alternative names for the antibody

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LEF1 antibody,|DKFZp586H0919 antibody|FLJ46390 antibody|LEF 1 antibody|LEF-1 antibody|Lef1 antibody|LEF1_HUMAN antibody|Lymphoid enhancer binding factor 1 antibody|Lymphoid enhancer-binding factor 1 antibody|T cell specific transcription factor 1 alpha antibody|T cell-specific transcription factor 1-alpha antibody|TCF 1 alpha antibody|TCF1 alpha antibody|TCF1-alpha antibody|TCF10 antibody|TCF1alpha antibody|TCF7L3 antibody|Transcription factor T cell specific 1 alpha antibody|Anti-LEF1 antibody [EP2030Y] (ab53293)
SCBT cat No: sc-81470|sc-374412|sc-374522|sc-8592|sc-28687|sc-8591|sc-56722|

 

Name

LEF-1 (phospho Ser42) Polyclonal Antibody

Catalogue No.

STJ90534

Reactivity

Human, Mouse, Rat

Specificity

Phospho-LEF-1 (S42) Polyclonal Antibody detects endogenous levels of LEF-1 protein only when phosphorylated at S42.

Immunogen

Synthesized phospho-peptide derived from LEF-1 (phospho Ser42) at AA range 20-100

Host

Rabbit

Applications

WB, ELISA

Recommended dilution

WB 1:500-1:2000; ELISA 1:5000;

Clonality

Polyclonal

Conjugation

Unconjugated

Isotype

IgG

Molecular weight

55 kDa

Formulation

LEF-1 (phospho Ser42) Antibody was tube-contained. Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration

1 mg/ml

Purification

LEF-1 (phospho Ser42) Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage

-20 Celsius degree. Avoid repeated freeze/thaw cycles.

Alternative antibody names

Lymphoid enhancer-binding factor 1 antibody, LEF-1 antibody, T cell-specific transcription factor 1-alpha antibody, TCF1-alpha antibody

Database links

Human UniProt/Swiss-Prot:Q9UJU2;Mouse UniPort/Swiss-Prot: P27782;Rat UniProt/Swiss-Port: Q9QXN1;Human Entrez Gene: 51176;Mouse Entrez Gene: 16842;Rat Entrez Gene: Rn.228611

Protein names

Lymphoid enhancer-binding factor 1 , LEF-1 , T cell-specific transcription factor 1-alpha , TCF1-alpha

Protein function

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Protein tissue specificity

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

Protein sequence and domain

Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. / Belongs to the TCF/LEF family. / Contains 1 HMG box DNA-binding domain.

Protein post-translational modifications

Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.

Protein cellular localization

Nucleus

Note

AntibodyPlus can customize LEF-1 (phospho Ser42) Antibody according to your requirement, including bulk product size,etc. Please contact info@antibodyplus.com. AntibodyPlus provide antibody trial sample for your own antibody validation and collects antibody reviews.

Supplier

St John’s Laboratory Ltd.

Product type

Primary antibody

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